Avidin D-HRP Avidin-D is a deglycosylated avidin purified from egg white preferred for it's highly specific binding to Biotin

Avidin D-HRP: sc-516217

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Datasheets

    • 15 ml; sufficient for 150 slides
    • Pre-diluted and ready-to-use solution of Avidin-D conjugated to horseradish peroxidase (HRP)
    • Avidin-D is a deglycosylated avidin purified from egg white preferred for it's highly specific binding to Biotin
    • Recommended when using the Labeled Steptavidin Biotin (LSAB) staining method for immunoperoxidase staining of tissues (IHC) or cells (ICC)
    • Recommended for use with conventional species-specific biotinylated secondary antibodies
    • Store at 4° C
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Can this reagent be used to detect biotinylated secondaries such as mouse anti-goat IgG-B (sc-2489) in a biotin-boosted western blot? If so, what protocol do you recomend?

Asked by: ISegUr
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Tech Service 1
Date published: 2021-01-28

Can you use this for direct detection of biotinylated protein on Western Blot? Any recommendation of what dilution to start with?

Asked by: LizB
Thank you for your question. We have not tested this Avidin D-HRP for this application. This is provided as 15 ml of pre-diluted and ready-to-use solution of Avidin-D conjugated to horseradish peroxidase (HRP). We do not have any recommendation for how much to use for that application. This is something that would have to be determined experimentally.
Answered by: Technical Support
Date published: 2019-04-23
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