Anti-Atm Antibody (G-12) recommended for detection of Atm of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Atm Antibody (G-12): sc-377293

Anti-Atm Antibody (G-12) is rated 4.6 out of 5 by 21.
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Datasheets

    • Anti-Atm Antibody (G-12) is a mouse monoclonal IgG1 κ Atm antibody, cited in 18 publications, provided at 200 µg/ml
    • raised against amino acids 2581-2860 of Atm (ataxia telangiectasia protein) of human origin
    • Atm Antibody (G-12) is recommended for detection of Atm of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Atm Antibody (G-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Atm (G-12): sc-377293.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for Atm Antibody (G-12) for WB and IHC(P) applications. This reagent is now offered in a bundle with Atm Antibody (G-12) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Can Atm (G-12): sc-377293 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Germaine
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Atm (G-12): sc-377293 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from works well in WB Works well in WB in human colon carcinoma cells 1:2000 O/N
Date published: 2021-10-08
Rated 4 out of 5 by from Works well for WB Worked well at all concentrations 1:100-1:1000 using 1:5000 anti-mouse secondary. Will use again at 1:500
Date published: 2021-06-16
Rated 4 out of 5 by from Worked very nicely for WB 1:500 Used on 3-7% tris acetate gel, HiMark pre-stained protein ladder. Incubated overnight at 4 degrees celsius in 5% milk TBST.
Date published: 2018-03-22
Rated 5 out of 5 by from Good antibody I need an ATM antibody directed against the ATM COOH terminal domain.Used at dilution 1:500. Worked fine.
Date published: 2018-02-14
Rated 5 out of 5 by from Works well for western blotting anti-ATM (G-12) is a good choic for western blot assay. I will buy it again.
Date published: 2017-09-30
Rated 5 out of 5 by from Good antibody for WB This antibody is good,we get a nice bands in human cells in WB at 1:500.
Date published: 2017-09-13
Rated 5 out of 5 by from Great for WB Works great for WB at 1:1000 dilution in HeLa and 293T cells.
Date published: 2017-09-06
Rated 5 out of 5 by from good antibody Good results in U20S human cells. Does not work for murine cells. Used at 1:500 dilution.
Date published: 2017-07-27
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