Anti-ATF-5 Antibody (E-10) recommended for detection of ATF-5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-ATF-5 Antibody (E-10): sc-377168

Anti-ATF-5 Antibody (E-10) is rated 3.8 out of 5 by 5.
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Datasheets

    • Anti-ATF-5 Antibody (E-10) is a mouse monoclonal IgG1 κ ATF-5 antibody, cited in 4 publications, provided at 200 µg/ml
    • raised against amino acids 1-83 mapping at the N-terminus of ATF-5 of human origin
    • Anti-ATF-5 Antibody (E-10) is recommended for detection of ATF-5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-ATF-5 Antibody (E-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-377168 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ATF-5 (E-10): sc-377168.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for ATF-5 Antibody (E-10) for WB and IHC(P) applications. This reagent is now offered in a bundle with ATF-5 Antibody (E-10) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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I am using ATF-5 (E-10): sc-377168 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Specific for ATF5 with a close non-specific band Works nicely for western blotting of HeLa lysates at a 1:500 dilution at 4 degrees overnight, producing a clean, specific band at approximately 38kDa. However, there is a strong non-specific band that runs very close to the ATF5 band at just above 40kDa.
Date published: 2019-02-07
Rated 4 out of 5 by from Working on Western Blot I got this as a free trial sample. The antibody specifically recognised a band at the right size upon TG treatment in HeLa cells.
Date published: 2018-09-24
Rated 1 out of 5 by from Not specific Found a band of similar size when comparing WT and ATF5KO western blots.
Date published: 2018-03-28
Rated 5 out of 5 by from WB: Single strong band in human liver tissue WB: Single strong band in human liver tissue extract. -SCBT QC
Date published: 2015-04-16
Rated 5 out of 5 by from Produced excellent immunoperoxidase nuclear Produced excellent immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human uterine cervix tissue. -SCBT QC
Date published: 2015-03-23
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