Anti-ATF-3 Antibody (44C3a) recommended for detection of ATF-3 of human and rat origin by WB, IP, IF and IHC(P)

Anti-ATF-3 Antibody (44C3a): sc-81189

Anti-ATF-3 Antibody (44C3a) is rated 4.0 out of 5 by 5.
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Datasheets

    • Anti-ATF-3 Antibody (44C3a) is a mouse monoclonal IgG1 κ ATF-3 antibody, cited in 20 publications, provided at 100 µg/ml
    • raised against a recombinant protein corresponding to an internal region of ATF-3 of human origin
    • recommended for detection of ATF-3 of human and rat origin by WB, IP, IF and IHC(P)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ATF-3 (44C3a): sc-81189.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for ATF-3 Antibody (44C3a) for WB and IHC(P) applications. These reagents are now offered in bundles with ATF-3 Antibody (44C3a) (see ordering information below).
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If I need to use anti-ATF3 in mouse tissue origin, which antibody should I order? Actually, I would like to use ATF3 (H-19) or (C-19) but it has been discontinued and replaced by ATF-3 (44C3a). However, 44C3a is not suitable for mouse tissue.

Asked by: Anonymous
Thank you for your question. ATF-3 Antibody (A-8): sc-518032 is recommended for detection of ATF-3 of mouse, rat and human origin by WB, IP, IF and ELISA.
Answered by: Tech Support Europe
Date published: 2021-04-18

Hi, I would like to request a free sample of the ATF-3 antibody (44C3a, sc-81189). Best, Floris

Asked by: Floris Leenders
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-14

This antibody can be used for mouse tissue?

Asked by: ttta
Thank you for the question. THis clone is recommended for detection of ATF-3 of human and rat origin by WB, IP, IF and IHC(P). We do not recommend this antibody for tissue samples of mouse origin.
Answered by: Technical Support Europe
Date published: 2017-12-27

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 9
Date published: 2017-01-24
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Rated 3 out of 5 by from good feature I used the antibody in 1:200 dilution and the band is very clear in MCF7 cells
Date published: 2018-04-12
Rated 4 out of 5 by from Detects a band between 22-25 kDa Tested free sample. Gives a band at 22-25kDa (Not as in the picture associated with the product), minimal background
Date published: 2018-01-23
Rated 5 out of 5 by from Good choice for ATF-3 ATF-3 Antibody (44C3a) is suitable for human and rat and featured in publications used in both WB and IF applications.
Date published: 2017-01-05
Rated 4 out of 5 by from Great results in WB using 1:500 dilution Great results in WB using 1:500 dilution on 25 g total protein lysate (rabbit embryo). -SCBT Publication Review
Date published: 2015-04-19
Rated 4 out of 5 by from WB: clear WB: clear, singular band when tested on human recombinant ATF-3 fusion protein. -SCBT QC
Date published: 2013-10-22
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