ATF-3 Antibody (44C3a): sc-81189

4.0(5)

- ATF-3 Antibody (44C3a) is a mouse monoclonal IgG₁ κ ATF-3 antibody, cited in 24 publications, provided at 100 µg/ml
- raised against a recombinant protein corresponding to an internal region of ATF-3 of human origin
- recommended for detection of ATF-3 of human and rat origin by WB, IP, IF and IHC(P)
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ATF-3 (44C3a): sc-81189.
- m-IgG Fc BP-HRP and m-IgG₁ BP-HRP are the preferred secondary detection reagents for ATF-3 Antibody (44C3a) for WB and IHC(P) applications. These reagents are now offered in bundles with ATF-3 Antibody (44C3a) (see ordering information below).

References:

Molecular cloning of human CREB-2: an ATF/CREB transcription factor that can negatively regulate transcription from the cAMP response element. | Karpinski, BA., et al. 1992. Proc Natl Acad Sci U S A. 89: 4820-4. PMID: 1534408
Cross-family dimerization of transcription factors Fos/Jun and ATF/CREB alters DNA binding specificity. | Hai, T. and Curran, T. 1991. Proc Natl Acad Sci U S A. 88: 3720-4. PMID: 1827203
A cDNA for a human cyclic AMP response element-binding protein which is distinct from CREB and expressed preferentially in brain. | Kara, CJ., et al. 1990. Mol Cell Biol. 10: 1347-57. PMID: 2320002
Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers. | Hai, TW., et al. 1989. Genes Dev. 3: 2083-90. PMID: 2516827
Interaction of a common cellular transcription factor, ATF, with regulatory elements in both E1a- and cyclic AMP-inducible promoters. | Lin, YS. and Green, MR. 1988. Proc Natl Acad Sci U S A. 85: 3396-400. PMID: 2835770
Identification of a cyclic-AMP-responsive element within the rat somatostatin gene. | Montminy, MR., et al. 1986. Proc Natl Acad Sci U S A. 83: 6682-6. PMID: 2875459
Cyclic AMP-responsive DNA-binding protein: structure based on a cloned placental cDNA. | Hoeffler, JP., et al. 1988. Science. 242: 1430-3. PMID: 2974179

Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession Number	Protein Accession Number	OMIM™ Number
Human	ATF3	Entrez Gene ID: 467	1q32.3	XM_005273146, XM_011509579, NM_001030287, NM_001040619, NM_001206484, NM_001206486, NM_001206488, NM_001674, NM_001206485, NM_004024	UniProt ID: P18847	603148
Mouse	Atf3	Entrez Gene ID: 11910	1 H6	NM_007498	UniProt ID: Q60765	N/A

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ATF-3 Antibody (44C3a) has 24 citations in a variety of scientific publications.

Primary antibodies like Anti-ATF-3 Antibody (44C3a) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ATF-3 Antibody (44C3a) Specifications

Alternate Product Name

ATF3

Product Clone Name

44C3a

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₁ kappa light chain

Amino Acids

Epitope Target

ATF-3

Epitope Species

human

Product Reactivity/Detection

ATF-3

Species Reactivity/Detection

human, rat

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P)

Vialing Information

Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 1.0% stabilizer protein

Research Citations

24 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

Mouse IgG₁ Binding Proteins

m-IgG₁ BP-HRP: sc-525408 (Mouse IgG₁ binding protein conjugated to horseradish peroxidase)
m-IgG₁ BP-B: sc-533660 (Mouse IgG₁ binding protein conjugated to biotin)
m-IgG₁ BP-FITC: sc-533659 (Mouse IgG₁ binding protein conjugated to fluorescein isothiocyanate)
m-IgG₁ BP-CFL 488: sc-533661 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 488)
m-IgG₁ BP-CFL 555: sc-533662 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 555)
m-IgG₁ BP-CFL 594: sc-533663 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 594)
m-IgG₁ BP-CFL 647: sc-533664 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 647)
m-IgG₁ BP-CFL 680: sc-533665 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 680)
m-IgG₁ BP-CFL 790: sc-533666 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

N/A

Primary antibodies for non-mammalian targets are useful in detecting proteins of plant, viral, bacterial, avian, fish, yeast and invertebrates. Some examples include Drosophila, zebrafish, Xenopus, Arabidopsis, C. elegans, Clostridium, Campylobacter, Heliobacter pylori, Hepatitis, HIV, HPV, HSV, Influenza, Klebsiella, M. tuberculosis, Plasmodium, Pseudomonas, RSV, Saccharomyces cerevisiae, Salmonella, Schistosoma, Shigella and Staphylococcus.

Santa Cruz Biotechnology monoclonal antibodies can be used in assays including flow cytometry, immunoprecipitation, Western blot analysis, and immunostaining. Anti-ATF-3 Antibody (44C3a) is recommended for use in the following applications: WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), IHC(P) (Immunohistochemistry - Paraffin Embedded).

Mouse secondary antibodies, control sera and control immunoglobulins are also offered to be used in combination with our primary monoclonal antibodies.

Tissue extracts, nuclear extracts and whole cell lysates are used with Santa Cruz Biotechnology monoclonal antibodies for western blotting or immunoprecipitation positive controls. Nuclear extracts are ideal for western blot analysis or gel shift assays positive controls. Phospho-enriched whole cell lysates and tissue extracts are available for phospho-specific antibody positive controls.

Monoclonal secondary antibodies are offered in various conjugated forms, including: Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), and TRITC (tetramethyl rhodamine isothiocyanate) (R), for research applications like Flow Cytometry (FCM), Immunofluorescence (IF), Immunohistochemistry (IHC) staining and Western Blotting (WB). Not intended for diagnostic or therapeutic use.

Affinity purified immunoglobulins (IgGs)—conjugated and unconjugated—are available as negative controls for flow cytometry (FCM), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP) and Western Blotting (WB) applications.

Normal sera for immunohistochemistry (IHC) applications are available as neat serum for blocking reagents. The serum which is used should match the species of the secondary antibody.

The preferred ATF-3 antibody is ATF-3 Antibody (A-8): sc-518032.

If I need to use anti-ATF3 in mouse tissue origin, which antibody should I order? Actually, I would like to use ATF3 (H-19) or (C-19) but it has been discontinued and replaced by ATF-3 (44C3a). However, 44C3a is not suitable for mouse tissue.

Asked by: Anonymous

Thank you for your question. ATF-3 Antibody (A-8): sc-518032 is recommended for detection of ATF-3 of mouse, rat and human origin by WB, IP, IF and ELISA.

Answered by: Tech Support Europe

Date published: 2021-04-18

Hi, I would like to request a free sample of the ATF-3 antibody (44C3a, sc-81189). Best, Floris

Asked by: Floris Leenders

Thank you for your question. Please contact Technical Service for information on how to obtain a sample.

Answered by: SCBT Heidelberg Support

Date published: 2018-12-14

This antibody can be used for mouse tissue?

Asked by: ttta

Thank you for the question. THis clone is recommended for detection of ATF-3 of human and rat origin by WB, IP, IF and IHC(P). We do not recommend this antibody for tissue samples of mouse origin.

Answered by: Technical Support Europe

Date published: 2017-12-27

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero

Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.

Answered by: Technical Support 9

Date published: 2017-01-24

Rated 3 out of 5 by ccliu1 from good feature I used the antibody in 1:200 dilution and the band is very clear in MCF7 cells

Date published: 2018-04-12

Rated 4 out of 5 by h123westerns from Detects a band between 22-25 kDa Tested free sample. Gives a band at 22-25kDa (Not as in the picture associated with the product), minimal background

Date published: 2018-01-23

Rated 5 out of 5 by cjMara from Good choice for ATF-3 ATF-3 Antibody (44C3a) is suitable for human and rat and featured in publications used in both WB and IF applications.

Date published: 2017-01-05

Rated 4 out of 5 by MS from Great results in WB using 1:500 dilution Great results in WB using 1:500 dilution on 25 g total protein lysate (rabbit embryo). -SCBT Publication Review

Date published: 2015-04-19

Rated 4 out of 5 by LM from WB: clear WB: clear, singular band when tested on human recombinant ATF-3 fusion protein. -SCBT QC

Date published: 2013-10-22

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