Anti-ATF-1/ATF1 Antibody (C41-5.1) recommended for detection of ATF-1 p35 of mouse, rat and human origin by WB and IP

Anti-ATF-1/ATF1 Antibody (C41-5.1): sc-243

Anti-ATF-1/ATF1 Antibody (C41-5.1) is rated 4.8 out of 5 by 10.
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Datasheets

    • ATF-1/ATF1 Antibody (C41-5.1) is a mouse monoclonal IgA κ, cited in 104 publications, provided at 200 µg/ml
    • raised against recombinant ATF-1 of human origin
    • recommended for detection of ATF-1 p35 of mouse, rat and human origin by WB and IP
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-243 X, 200 µg/0.1 ml)
    • See ATF-1/ATF1 (25C10G): sc-270 for ATF-1/ATF1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ATF-1/ATF1 (C41-5.1): sc-243.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for ATF-1/ATF1 Antibody (C41-5.1). This work is in progress.
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When should the TransCruz reagent sc-243 X be used instead of the standard form sc-243?

Asked by: jerojero
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from It is fine. Used for WB to detect human cancers. Got the specific band, but too weak. Supersignal ECL is required.
Date published: 2020-08-13
Rated 4 out of 5 by from Specific for ATF1 in WB I overexpressed GFP-tagged ATF1, CREB and CREM in HEK293 cells. ATF1 C41-5.1 was specific to ATF1 in WB, as it recognised only GFP-ATF1 protein, but not GFP-CREB or GFP-CREM. Dilution 1:200 worked very well in WB with overexpressed ATF1 in HEK293 cells, but failed to recognize endogenous ATF1 and gave a very strong background signal in rat primary cortical neuron lysates.
Date published: 2018-08-23
Rated 5 out of 5 by from Great Antibody for WB This antibody is well-cited for a variety of applications including WB, IP, even ChIP. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-30
Rated 5 out of 5 by from Used this antibody for WB detection and it Used this antibody for WB detection and it worked well.
Date published: 2015-10-05
Rated 5 out of 5 by from Strong band in WB in HeLa nuclear extract Strong band in WB in HeLa nuclear extract. -SCBT QC
Date published: 2015-04-06
Rated 5 out of 5 by from Data published for WB Data published for WB, IP, and EMSA in rat cortical neuron. -SCBT Publication Review
Date published: 2015-03-31
Rated 5 out of 5 by from Published data for WB in mouse RAW264 Published data for WB in mouse RAW264.7 and human THP-1 cell lines. -SCBT Publication Review
Date published: 2015-03-27
Rated 5 out of 5 by from Publishable data for EMSA in human JEG Publishable data for EMSA in human JEG-3, JAR, Tera-2, and HeLa cells. -SCBT Publication Review
Date published: 2015-03-17
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