Anti-ATF-1/ATF1 Antibody (25C10G) is a mouse monoclonal IgG1 κ ATF-1/ATF1 antibody, cited in 100 publications, provided at 200 µg/ml
raised against amino acids 39-271 mapping within the DNA binding and dimerization domain of ATF-1 of human origin
Anti-ATF-1/ATF1 Antibody (25C10G) is recommended for detection of ATF-1 p35, CREB-1 p43 and CREM-1 of mouse, rat and human origin by WB, IP, IF and IHC(P)
Anti-ATF-1/ATF1 Antibody (25C10G) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
TransCruz reagent for Gel Supershift and ChIP applications (sc-270 X, 200 µg/0.1 ml)
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ATF-1/ATF1 (25C10G): sc-270.
m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for ATF-1/ATF1 Antibody (25C10G) for WB and IHC(P) applications. These reagents are now offered in bundles with ATF-1/ATF1 Antibody (25C10G) (see ordering information below).
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
ATF-1 Antibody (25C10G) is a high quality monoclonal ATF-1 antibody (also designated ATF1 antibody, activating transcription factor 1 antibody or TREB36 antibody) suitable for the detection of the ATF-1 protein of mouse, rat and human origin. ATF-1 Antibody (25C10G) is available as both the non-conjugated anti-ATF-1 antibody form, as well as multiple conjugated forms of anti-ATF-1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Eukaryotic gene transcription is regulated by sequence-specific transcription factors that bind modular cis acting promoter and enhancer elements. The ATF/ CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-1 is shown to play a key role in the induction of NOX1. ATF-1 binds the cAMP response element (CRE) and mediates PKA-induced stimulation of CRE-reporter genes. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CRE-dependent transcription. Phosphorylation of ATF-2 at Thr 69 and Thr 71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr 69 and Thr 71 to prolong the half-life of ATF-2. ATF-2 functions as a histone acetyltransferase (HAT) and acetylates Histones H2B and H4 specifically in vitro.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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