Anti-ATF-1/ATF1 Antibody (25C10G) recommended for detection of ATF-1 p35, CREB-1 p43 and CREM-1 of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-ATF-1/ATF1 Antibody (25C10G): sc-270

Anti-ATF-1/ATF1 Antibody (25C10G) is rated 4.8 out of 5 by 11.
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Datasheets

    • Anti-ATF-1/ATF1 Antibody (25C10G) is a mouse monoclonal IgG1 κ ATF-1/ATF1 antibody, cited in 100 publications, provided at 200 µg/ml
    • raised against amino acids 39-271 mapping within the DNA binding and dimerization domain of ATF-1 of human origin
    • Anti-ATF-1/ATF1 Antibody (25C10G) is recommended for detection of ATF-1 p35, CREB-1 p43 and CREM-1 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • Anti-ATF-1/ATF1 Antibody (25C10G) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-270 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ATF-1/ATF1 (25C10G): sc-270.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for ATF-1/ATF1 Antibody (25C10G) for WB and IHC(P) applications. These reagents are now offered in bundles with ATF-1/ATF1 Antibody (25C10G) (see ordering information below).
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When should the TransCruz reagent sc-270 X be used instead of the standard form sc-270?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from Recognizes all CREB family members in WB I overexpressed GFP-tagged ATF1, CREB and CREM in HEK293 cells. ATF1 25C10G (dilution 1:200) recognized GFP-ATF1, but was not specific to ATF1 in WB, as it recognised all the GFP-tagged CREB family members.
Date published: 2018-08-23
Rated 5 out of 5 by from Excellent choice for IHC(P) and WB Very well rounded antibody for IHC on paraffin sections with nice specific clean staining. Also great for WB and IF. All around, a great choice!
Date published: 2017-02-01
Rated 5 out of 5 by from improve The antibody properties are easier to observe, the overall characteristics of dilution preservation intact, than the previous generation has a greater improvement
Date published: 2017-01-17
Rated 5 out of 5 by from good product The product is good. The result show that the antibody expressed in lung cancer.
Date published: 2016-11-29
Rated 5 out of 5 by from Great This production is very great with high quanlity and high specificity.
Date published: 2016-11-19
Rated 5 out of 5 by from Publishable data for WB in human transfected Saos Publishable data for WB in human transfected Saos-2 and U-2 OS cells.
Date published: 2015-04-22
Rated 5 out of 5 by from Good band in WB in KNRK nuclear extract Good band in WB in KNRK nuclear extract.-SCBT QC
Date published: 2015-03-12
Rated 5 out of 5 by from Produced great immunoperoxidase nuclear staining Produced great immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-03-03
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