Anti-ASC/TMS1/PYCARD Antibody (B-3) recommended for detection of ASC of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-ASC/TMS1/PYCARD Antibody (B-3): sc-514414

Anti-ASC/TMS1/PYCARD Antibody (B-3) is rated 4.8 out of 5 by 40.
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Datasheets

    • Anti-ASC/TMS1/PYCARD Antibody (B-3) is a mouse monoclonal IgG1 κ ASC/TMS1/PYCARD antibody, cited in 122 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 174-195 at the C-terminus of ASC of human origin
    • Anti-ASC/TMS1/PYCARD Antibody (B-3) is recommended for detection of ASC of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-ASC/TMS1/PYCARD Antibody (B-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ASC/TMS1/PYCARD (B-3): sc-514414.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ASC/TMS1/PYCARD Antibody (B-3) for WB and IHC(P) applications. These reagents are now offered in bundles with ASC/TMS1/PYCARD Antibody (B-3) (see ordering information below).
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Hi, I would like to know what is the difference between ASC (B3) sc-514414 and ASC (D3) sc-514559 for IHC choice. Thanks.

Asked by: yzmonica
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Tech Service 1
Date published: 2021-02-04

Has anyone bought this antibody recently?How does it work?

Asked by: a mei
Thank you for your question. ASC Antibody (B-3): sc-514414 detects ASC of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. You can also check out the publications we list on this antibody's product page. If you are looking for a specific application, feel free to contact our technical service team and we will check our databases for matching publications.
Answered by: Tech Support Europe
Date published: 2020-11-23

Hi, Have any of your ASC antibodies been confirmed to detect ASC Speck specifically in human samples (supernatants, plasma, CSF etc)? Many thanks 

Asked by: Annon
Thank you for your question. Yes, this antibody will detect ASC in human samples. We have many publications of it being used in human samples, you can find them above in the Product Citations section.
Answered by: Tech Service
Date published: 2021-05-19

이 안티바디 secondary가 무엇인가요?

Asked by: hyosun444
산타크루즈바이오테크놀러지에 문의 주셔서 감사합니다. 아래는 우리 회사 추천하는 이차 항체 웹 링크입니다. https://www.scbt.com/scbt/browse/Anti-Mouse-IgG-Binding-Protein-Secondaries/_/N-130b1ry다른 문제 있으시면 전화:86 21-60936350 이메일: [email protected] 또는 온라인 문의로 문의하시면 됩니다. Thank you for your question. please contact our Technical Service Department at email [email protected],call us directly at (86 21) 6093-6350, or contact us by live chat.
Answered by: Technical Support 8
Date published: 2018-12-21

Hi, there were references concerning the N-15 antibody and flow cytometry usage for intracellular staining of ASC, especially for speck formation. Do you have any data or customer feedback indicating that the B-3 antibody would work for this application?

Asked by: PierreM
Thank you for your question. This antibody sc-514414 is recommended for detection of ASC of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. We currently do not have data for an application in FCM for this product.
Answered by: SCBT Heidelberg Support
Date published: 2018-11-27

FREE 10 µg sample of ASC (B-3): sc-514414 Conjugated to Alexa Fluor 488 How do I get about getting a free sample of the above?

Asked by: ShellyP
Thank you very much for your question. Unfortunately, we don't offer samples of conjugated antibodies. If you need any other sample, please send us an email to [email protected] with the following information: - complete address for shipping - telephone, fax number and e-mail address - a contact person the products should be addressed to - catalogue number and product description Remember once you try the sample, you can write a review in our web about it, indicating all the information you think might be useful for other researchers.
Answered by: Technical Support Europe
Date published: 2018-03-05

Is ASC (B-3): sc-514414 monoclonal antibody available directly conjugated to agarose for IP use?

Asked by: TinTin
Yes! This antibody is available conjugated to agarose (catalog number: sc-514414 AC) for use in IP. It’s also available conjugated to HRP (sc-514414 HRP), phycoerythrin (sc-514414 PE), fluorescein (sc-514414 FITC), Alexa Fluor® 488 (sc-514414 AF488) or Alexa Fluor® 647 (sc-514414 AF647) should any other conjugations be desired.
Answered by: Technical Support 7
Date published: 2017-01-18
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Rated 4 out of 5 by from 条带比较清晰单一 做的NLRP3炎性小体,在肿瘤细胞和神经细胞上分别使用了该抗体,每次都有条带,并且背景比较少,条带清晰!
Date published: 2020-12-31
Rated 5 out of 5 by from Robust Antibody This antibody specific and sensitive. I got very intense signal using ECL for only 15ug of total protein.
Date published: 2018-12-18
Rated 5 out of 5 by from Great antibody! I received this antibody as a sample and performed WB on adenovirus infected cells. Specificity was amazing and signal more than beautiful. I will definitely continue using it for my research.
Date published: 2018-06-06
Rated 5 out of 5 by from WB We highly recommended this antibody for WB assay.It give us expected size at 24kDa.Human breast cancer tissue extracts been used,very clean and specific bands.
Date published: 2018-05-26
Rated 4 out of 5 by from ASC antibodies (B-3)Sc-514414 I bought this antibody a month ago and tried it in the Western Blot with lens and lung tissues.
Date published: 2018-02-12
Rated 5 out of 5 by from Works great in human samples I have tested this antibody in HCAEC by western blot. Its works great. This antibody is very specific and the membrane is clean without any background. Highly recommended.
Date published: 2018-02-06
Rated 4 out of 5 by from It works well! I bought this antibody one month ago and used it to monitor inflammasome activation in THP-1 macrophages with treatment. It can stain the LPS+ATP control-treated cells, but not untreated cells. SO it works well and I recommend it.
Date published: 2018-02-03
Rated 5 out of 5 by from excelente resultado Testé este anticuerpo para WB en tejido de conejo inflamado y tenemos reconocimiento específico de la proteina.
Date published: 2017-12-12
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