Arp2 Antibody (E-2)recommended for detection of Arp2 of mouse, rat and human origin by WB, IP, IF and ELISA

Arp2 Antibody (E-2): sc-137250

Arp2 Antibody (E-2) is rated 5.0 out of 5 by 3.
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Datasheets
    • Arp2 Antibody (E-2) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 311-394 of Arp2 of human origin
    • recommended for detection of Arp2 of mouse, rat and human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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What is the recommended fixation method for immunofluorescence staining with Arp2 (E-2): sc-137250 monoclonal antibody?

Asked by: jerojero
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good signal observed in WB using human Arp2 Good signal observed in WB using human Arp2 transfected 293 and HeLa whole cell lysates. -SCBT QC
Date published: 2015-04-07
Rated 5 out of 5 by from Great antibody for western blot; clean bands Great antibody for western blot; clean bands in 293T, C32, NIH/3T3 and RAW 264.7 whole cell lysates. -SCBT QC
Date published: 2015-03-12
Rated 5 out of 5 by from Excellent immunofluorescence cytoplasmic Excellent immunofluorescence cytoplasmic staining in methanol-fixed NIH/3T3 cells. -SCBT QC
Date published: 2015-01-20
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