Anti-ARHGAP21 Antibody (E-10) recommended for detection of ARHGAP21 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-ARHGAP21 Antibody (E-10): sc-390145

Anti-ARHGAP21 Antibody (E-10) is rated 5.0 out of 5 by 2.
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Datasheets

    • Anti-ARHGAP21 Antibody (E-10) is a mouse monoclonal IgG1 ARHGAP21 antibody, cited in 2 publications, provided at 200 µg/ml
    • raised against amino acids 241-540 mapping within an internal region of ARHGAP21 of human origin
    • Anti-ARHGAP21 Antibody (E-10) is recommended for detection of ARHGAP21 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-ARHGAP21 Antibody (E-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ARHGAP21 (E-10): sc-390145.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for ARHGAP21 Antibody (E-10) for WB and IHC(P) applications. These reagents are now offered in bundles with ARHGAP21 Antibody (E-10) (see ordering information below).
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Good morning I would like to know the amminoacidic sequence recognized by your ARHGAP21 antibody (sc-390145). Have you performed an epitope map of the antibody? I need a antibody recognizing before aa 438... Thank you

Asked by: Simcopp
Thank you for your question. This antibody is specific for Bcl-2. It was raised against amino acids 241-540 mapping within an internal region of ARHGAP21 of human origin (accession#Q5T5U3), the exact epitope has not been mapped. If you have any further questions, please contact Technical Service.
Answered by: Tech Service
Date published: 2019-04-02

I am using ARHGAP21 (E-10): sc-390145 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Trav11
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Worked well Used at a 1:250 dilution for a western blot with NRVMs and it gave a nice, clean band.
Date published: 2017-06-18
Rated 5 out of 5 by from Produced positive Western blot data of ARHGAP21 Produced positive Western blot data of ARHGAP21 expression in T98G whole cell lysate. -SCBT QC
Date published: 2013-05-13
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