APRIN Antibody (F-9): sc-518248

APRIN Antibody (F-9) is a mouse monoclonal IgM antibody that detects an epitope mapping between amino acids 1254-1273 of APRIN of human origin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). APRIN monoclonal antibody (F-9) is available in a non-conjugated form, allowing for versatile experimental use. APRIN, also known as androgen-induced proliferation inhibitor, Androgen Shutoff 3 (AS3), or PDS5 regulator of cohesion maintenance homolog B (PDS5B), plays a crucial role in regulating androgen-dependent growth arrest in prostate cells, specifically mediating cell cycle arrest in the G0/G1 phase of prostate epithelial cells. APRIN (F-9) antibody recognizes a protein that is highly conserved and features a nuclear localization sequence near its C-terminal, a DNA-binding domain, a coiled-coil domain, a leucine zipper, and a protein kinase domain, which are essential for cellular processes. APRIN localization in the nucleus suggests involvement in transcriptional regulation and potential interactions with other nuclear proteins. APRIN expression in smooth muscle stromal cells and both basal and luminal epithelial cells highlights significance in prostate tissue homeostasis. A loss of the gene encoding APRIN has been strongly correlated with the development of prostate cancer, making anti-APRIN antibody (F-9) a valuable tool for studying its role in normal physiology and disease contexts.

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APRIN Antibody (F-9) References:

1. Early gene expression during androgen-induced inhibition of proliferation of prostate cancer cells: a new suppressor candidate on chromosome 13, in the BRCA2-Rb1 locus.  |  Geck, P., et al. 1999. J Steroid Biochem Mol Biol. 68: 41-50. PMID: 10215036
2. Spo76p is a conserved chromosome morphogenesis protein that links the mitotic and meiotic programs.  |  van Heemst, D., et al. 1999. Cell. 98: 261-71. PMID: 10428037
3. Androgen-induced proliferative quiescence in prostate cancer cells: the role of AS3 as its mediator.  |  Geck, P., et al. 2000. Proc Natl Acad Sci U S A. 97: 10185-90. PMID: 10963680
4. Polymorphism and allelic loss at the AS3 locus on 13q12-13 in esophageal squamous cell carcinoma.  |  Harada, H., et al. 2001. Int J Oncol. 18: 1003-7. PMID: 11295048
5. Mechanism of androgen action on cell proliferation: AS3 protein as a mediator of proliferative arrest in the rat prostate.  |  Maffini, MV., et al. 2002. Endocrinology. 143: 2708-14. PMID: 12072405
6. Significant contribution of germline BRCA2 rearrangements in male breast cancer families.  |  Tournier, I., et al. 2004. Cancer Res. 64: 8143-7. PMID: 15548676
7. Exogenous fibroblast growth factors maintain viability, promote proliferation, and suppress GADD45alpha and GAS6 transcript content of prostate cancer cells genetically modified to lack endogenous FGF-2.  |  Shain, SA. 2004. Mol Cancer Res. 2: 653-61. PMID: 15561781
8. Androgen receptor signaling and vitamin D receptor action in prostate cancer cells.  |  Murthy, S., et al. 2005. Prostate. 64: 362-72. PMID: 15754350
9. Sororin, a substrate of the anaphase-promoting complex, is required for sister chromatid cohesion in vertebrates.  |  Rankin, S., et al. 2005. Mol Cell. 18: 185-200. PMID: 15837422
10. APRIN is a cell cycle specific BRCA2-interacting protein required for genome integrity and a predictor of outcome after chemotherapy in breast cancer.  |  Brough, R., et al. 2012. EMBO J. 31: 1160-76. PMID: 22293751
11. Overexpression of APRIN inhibits differentiation and proliferation and promotes apoptosis in P19 embryonal carcinoma cells.  |  Zhou, X., et al. 2013. Mol Biol Rep. 40: 491-5. PMID: 23054015
12. Downregulation of APRIN expression increases cancer cell proliferation via an interleukin-6/STAT3/cyclin D axis.  |  Sohn, MS., et al. 2021. Oncol Lett. 21: 55. PMID: 33281966

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-APRIN Antibody (F-9) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-APRIN Antibody (F-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

APRIN Antibody (F-9) Specifications

Alternate Product Name: APRIN

Product Clone Name: F-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 1254-1273

Epitope Target: APRIN

Epitope Species: human

Product Reactivity/Detection: APRIN

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for APRIN Antibody (F-9): N/A