apoOL Antibody (G-6)recommended for detection of apoOL of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

apoOL Antibody (G-6): sc-390958

apoOL Antibody (G-6) is rated 4.4 out of 5 by 5.
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Datasheets

    • apoOL Antibody (G-6) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 239-262 near the C-terminus of apoOL of human origin
    • apoOL Antibody (G-6) is recommended for detection of apoOL of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine and bovine
    • apoOL Antibody (G-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-390958 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of apoOL (G-6): sc-390958.
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What application is the blocking peptide sc-390958 P appropriate for?

Asked by: jenni
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Good for IF Tested for IF on HeLa at 1:100 dilution. Good labeling of mitochondria (co-localization with another mitochondrial marker), albeit with some background signal.
Date published: 2017-10-30
Rated 4 out of 5 by from Good antibody! This antibody works well in Western Blot, is better on PVDF membrane however.
Date published: 2017-09-28
Rated 4 out of 5 by from SC-390958 , good performance , hCT116 /hela/ GP2-293 /H1299 /HELA endogenous apool , positive resulft without unspecific band .
Date published: 2016-12-08
Rated 5 out of 5 by from Excellent immunoperoxidase cytoplasmic and Excellent immunoperoxidase cytoplasmic and nuclear staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-04-20
Rated 5 out of 5 by from Good Good, strong band in WB in mouse apoOL transfected 293T, T24 and HeLa whole cell lysates. -SCBT QC
Date published: 2015-03-25
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