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apoL1 Antibody (A-3) | SCBT - Santa Cruz Biotechnology
apoL1 Antibody (A-3) recommended for detection of apoL1 of human origin by WB, IP, IF and ELISA

apoL1 Antibody (A-3): sc-390440

apoL1 Antibody (A-3) is rated 4.5 out of 5 by 4.
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Datasheets

    • apoL1 Antibody (A-3) is a mouse monoclonal IgG1 κ apoL1 antibody, cited in 3 publications, provided at 200 µg/ml
    • raised against amino acids 201-285 mapping within an internal region of apoL1 of human origin
    • apoL1 Antibody (A-3) is recommended for detection of apoL1 of human origin by WB, IP, IF and ELISA
    • Anti-apoL1 Antibody (A-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of apoL1 (A-3): sc-390440.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for apoL1 Antibody (A-3) for WB applications. These reagents are now offered in bundles with apoL1 Antibody (A-3) (see ordering information below).
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What is the recommended starting dilution for Western blot for sc-390440: apoL1 (A-3) monoclonal antibody?

Asked by: TinTin
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 4 out of 5 by from Effect of APOL1 disease risk variants on APOL1 gen Our present study focuses on the impact of these variants on APOL1 mRNA transcription and translation. APOL1 plasmids (EV, G0, G1 and G2) were transfected into human embryonic kidney (HEK) 293T cells. APOL1 variant expression was observed to be significantly lower than that of APOL1G0. Podocyte cell lines stably expressing APOL1 transgenes also showed lower levels of APOL1 expression of APOL1 variants (G1 and G2) compared with APOL1G0 by Western blotting and FACS analysis. APOL1 mRNA transcript stability was tested after actinomycin D pulsing; APOL1G1 and APOL1G2 mRNAs transcript decayed 10-15% and 15-20% (within a period of 0.5-3 h) respectively. Our data suggest that down-regulated APOL1 protein expression in APOL1 variants is due to compromised transcription and decay of the APOL1 variant transcripts.
Date published: 2017-08-11
Rated 5 out of 5 by from It is good! I have been used this antibody for incubate the membrane, the result is good!
Date published: 2017-07-25
Rated 5 out of 5 by from worked well in western blot We used this antibody for western blot and got a band around the correct molecular weight
Date published: 2017-01-16
Rated 4 out of 5 by from Good specificity and signal strength with Good specificity and signal strength with little background in human plasma. -SCBT QC
Date published: 2014-06-19
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