apoE Antibody (WU E-4)recommended for detection of apoE of mouse, rat and human origin by WB, IP, IF and IHC(P)

apoE Antibody (WU E-4): sc-53570

apoE Antibody (WU E-4) is rated 4.7 out of 5 by 7.
  • y_2019, m_12, d_14, h_13
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.4
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_7
  • loc_en_US, sid_53570, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 91ms
  • REVIEWS, PRODUCT
5
5
4
2
3
0
2
0
1
0

Datasheets

    • apoE Antibody (WU E-4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against ApoE of human origin
    • apoE Antibody (WU E-4) is recommended for detection of apoE of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • apoE Antibody (WU E-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of apoE (WU E-4): sc-53570.
Submit a review for this product and receive 15 CruzCredits

Which APOE epitope is recognized by this antibody (WU E-4)?

Asked by: Suzie
Thank you for your question. The exact epitope for this monoclonal antibody has not yet been epitope mapped, so we do not have this information. It was raised against purified human ApoE of human origin. Please contact our Technical Service Department and we would be glad to discuss the reactivity of this antibody. You can reach us by email at scbt@scbt.com, or use the live chat on our website.
Answered by: Tech Service
Date published: 2017-06-13

Was bedeutet FL bei der Beschreibung des Epitops für diesen AK?

Asked by: Suzie
Vielen Dank für Ihre Anfrage und Ihr Interesse an diesem Produkt. Der apoE Antikörper (WU E-4): sc-53570 wurde gegen das humane ApoE Volllängeprotein gezogen. Die Bezeichnung FL steht in diesem Fall für "full length". Für weitere Informationen wenden Sie sich bitte an unseren Technischen Service in unserem Europäischen Supportbüro in Heidelberg. Sie erreichen uns telefonisch unter 06221 4503 0, per email an: europe@scbt.com oder per Live-Chat auf unserer Webseite www.scbt.com Wir freuen uns Ihnen weiterzuhelfen.
Answered by: Technical Support Europe
Date published: 2017-05-18

What is the recommended starting dilution for immunostaining with apoE (WU E-4): sc-53570 monoclonal antibody?

Asked by: jenni
Thank you for your question. The recommended starting dilution for immunostaining is 1:50 with a dilution range of 1:50-1:500. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 5
Date published: 2017-01-20
  • y_2019, m_12, d_14, h_13CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.4
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_en_US, sid_53570, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 92ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Excellent detection of apoE by WB and ICC This antibody showed excellent signal for all three major isoforms of apoE by WB and ICC in murine, rodent and human cell lines. It appears to be specific for the N-terminus of apoE (confirmed by western blotting data using cell lines overexpressing truncated variants of apoE protein).
Date published: 2018-04-06
Rated 4 out of 5 by from Detection by Western Blot Clean signal for endogenous ApoE from HepG2 cells as well as recombinant ApoE down to 5 ng.
Date published: 2018-04-05
Rated 5 out of 5 by from Wow !! Superb product Outstanding product for both western blot and IHC staining applications
Date published: 2017-02-04
Rated 5 out of 5 by from Reliable and specific This antibody is great for Western blot detection of apoE, nice strong bands every time!
Date published: 2016-12-21
Rated 4 out of 5 by from Produced great immunoperoxidase cytoplasmic Produced great immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human testis tissue. -SCBT QC
Date published: 2015-05-12
Rated 5 out of 5 by from Works well for WB with Hep G2 whole cell lysate Works well for WB with Hep G2 whole cell lysate. -SCBT QC
Date published: 2015-05-06
Rated 5 out of 5 by from Sharp signal obtained in WB with human platelet Sharp signal obtained in WB with human platelet extract. -SCBT QC
Date published: 2015-03-10
  • y_2019, m_12, d_14, h_13
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.4
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_7
  • loc_en_US, sid_53570, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 4ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2019 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.