apoE Antibody (F-9)recommended for detection of apoE of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

apoE Antibody (F-9): sc-390925

apoE Antibody (F-9) is rated 4.7 out of 5 by 10.
  • y_2019, m_12, d_3, h_16
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.3
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_10
  • loc_en_US, sid_390925, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 98ms
  • REVIEWS, PRODUCT
5
8
4
1
3
1
2
0
1
0

Datasheets

    • apoE Antibody (F-9) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 31-253 mapping within an internal region of apoE of human origin
    • apoE Antibody (F-9) is recommended for detection of apoE of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • apoE Antibody (F-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of apoE (F-9): sc-390925.
Submit a review for this product and receive 15 CruzCredits

apoE F-9 has replaced M-20. However, M-20 binds on the C-terminus of apoE, while F-9 binds within an internal region of apoE. Therefore, by F-9 we cannot detect apoE fragments previously detected with M-20. Any suggestion for another replacement?

Asked by: Aggelika
Thank you for your question. You can find all antibodies we offer against apoE by following this link: https://www.scbt.com/browse/apoe-Antik%C3%B6rper/_/N-pr9qxd?&_requestid=4836 apoE Antibody (A1.4): sc-13521, for instance, is specific against amino acids 126-191 of apoE of human origin.
Answered by: Tech Support Europe
Date published: 2019-11-22

Does this APOE antibody (F-9) have different affinity to different APOE isoforms (E2, E3, and E4)?

Asked by: CSYang
Thank you for your question. We have not performed any testing with this apoE Antibody (F-9): sc-390925 to determine reactivity with, or affinity for the different apoE isoforms, E2, E3 and E4.
Answered by: Technical Support
Date published: 2017-05-17

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 14
Date published: 2017-01-26

The epitope mapping does not make sense - this protein is 319 aa long if including the signaling peptide. Please could you clarify this epitope sequence?

Asked by: Jade
Thank you for your question, and for bringing this discrepancy to our attention. After reviewing the product, we have made a typo in the amino acid range listed as the immunogen for this antibody and incorrectly listed the range as 31-353. The correct range is 31-253. This antibody, apoE Antibody (F-9): sc-390925, is a mouse monoclonal antibody raised against a recombinant protein corresponding to amino acids 31-253 of apoE of human origin.
Answered by: Technical Support
Date published: 2017-03-02
  • y_2019, m_12, d_3, h_16CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.3
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_4
  • loc_en_US, sid_390925, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 99ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Research associate It works very well in western blotting, strong signal and low back grand..
Date published: 2018-11-29
Rated 5 out of 5 by from works for both mouse and human APOEs We tried this APOE ab as a sample. The bands were pretty clear and no band in our KO mouse Brian samples or human ApoE KO lines.
Date published: 2018-04-05
Rated 5 out of 5 by from dilution 1:200 Antibody can detect apoE by WB, the band is clear, antibody is very good
Date published: 2017-09-07
Rated 5 out of 5 by from very good antibody Clean WB blot with single strong band in human liver tissue extract and 293T whole cell lysate.
Date published: 2017-08-11
Rated 3 out of 5 by from apoE WB:there is a clear bands in mouse liver tissue,and used 1:500 dilution
Date published: 2017-08-09
Rated 4 out of 5 by from Works well for IF in fetal RPE cells Good signal in differentiated fetal RPE cells by immunofluorescence.
Date published: 2017-04-20
Rated 5 out of 5 by from Strong signal Very strong and specific signal using apoE Antibody (F-9) by WB and IHCP
Date published: 2017-01-24
Rated 5 out of 5 by from Exceptional immunoperoxidase cytoplasmic Exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human testis tissue. -SCBT QC
Date published: 2015-05-03
  • y_2019, m_12, d_3, h_16
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.3
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_10
  • loc_en_US, sid_390925, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 34ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2019 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.