apoE Antibody (E-8)recommended for detection of precursor and mature apoE of human origin by WB, IP, IF and ELISA

apoE Antibody (E-8): sc-393302

apoE Antibody (E-8) is rated 5.0 out of 5 by 3.
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Datasheets

    • apoE Antibody (E-8) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 293-318 near the C-terminus of apoE of human origin
    • apoE Antibody (E-8) is recommended for detection of precursor and mature apoE of human origin by WB, IP, IF and ELISA
    • apoE Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to biotin for WB, IHC(P) and ELISA
    • blocking peptide, sc-393302 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of apoE (E-8): sc-393302.
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What application is the blocking peptide sc-393302 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Specific to human apoE Great affinity for human apoE in Western Blot, shown to be selective and not reactive with mouse apoE.
Date published: 2017-01-25
Rated 5 out of 5 by from Clear Clear, strong band in WB in Hep G2 whole cell lysate and and human liver tissue extract. Antibody lacked reactivity in mouse liver tissue extract. -SCBT QC
Date published: 2015-02-12
Rated 5 out of 5 by from Produced positive Western blot data of apoE Produced positive Western blot data of apoE expression in Hep G2 whole cell lysate and mouse liver and human liver tissue extracts. -SCBT
Date published: 2013-12-18
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