apoE Antibody (A1.4)recommended for detection of apoE of human origin by WB, IP, IF and IHC(P)

apoE Antibody (A1.4): sc-13521

apoE Antibody (A1.4) is rated 4.5 out of 5 by 11.
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Datasheets

    • apoE Antibody (A1.4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 126-191 of apoE of human origin
    • apoE Antibody (A1.4) is recommended for detection of apoE of human origin by WB, IP, IF and IHC(P)
    • apoE Antibody (A1.4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of apoE (A1.4): sc-13521.
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I have run out of my usual goat anti-mouse secondary antibody. What do you recommend I use for detection in my WB?

Asked by: cjMara
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 10
Date published: 2017-01-13

Can I use apoE Antibody (A1.4): sc-13521 for detection in mouse samples?

Asked by: cjMara
We have not yet validated this antibody for use in mouse samples, it is only currently validated for use in human. We would recommend one of our alternative clones, sc-390925: apoE (F-9) which is recommended for use in mouse.
Answered by: Technical Support 10
Date published: 2017-01-13
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Rated 1 out of 5 by from Does not work for FCM The apoE antibody does not work for FCM with HFF cells.
Date published: 2018-11-15
Rated 4 out of 5 by from Great antibody for plasma I tried this antibody for mouse and human plasma sample. Works great !
Date published: 2018-03-06
Rated 4 out of 5 by from Good product The result shows positive signal in A549 whole cell lysates. Works very well in WB at 1:500 dilution, satisfied result.
Date published: 2017-05-17
Rated 5 out of 5 by from Wow !! Outstanding Superb IF and IHC staining data; also great WB results
Date published: 2017-01-23
Rated 5 out of 5 by from Good for staining This apoE antibody shows good results for staining.
Date published: 2017-01-14
Rated 5 out of 5 by from detect the PD biomerker(ApoE) in hyperlipidemia pa I bought this antibody several months aga. It can detect the PD biomerker(ApoE) in hyperlipidemia patients. It works very specific to the target as the capture antibody in ELISA
Date published: 2016-12-17
Rated 5 out of 5 by from Good publishable data in WB in human HepG2 cells Good publishable data in WB in human HepG2 cells. -SCBT Publication Review
Date published: 2015-04-12
Rated 5 out of 5 by from Good specificity in WB in human platelet extract Good specificity in WB in human platelet extract. -SCBT QC
Date published: 2015-03-04
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