apoA-V Antibody (1G5G9) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
raised against purified truncated recombinant human apoA-V
recommended for detection of apoA-V of human origin by WB and IP
m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for apoA-V Antibody (1G5G9) for WB applications. These reagents are now offered in bundles with apoA-V Antibody (1G5G9) (see ordering information below).
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Apolipoproteins are protein components of plasma lipoproteins. The human apoA-I gene encodes a single chain, 243 amino acid protein which promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A-I is the major protein component of high density lipoprotein (HDL) in the plasma. It can function as a cofactor for lecithin cholesterolacyltransferase (LCAT), which is responsible for the formation of most plasma cholesteryl esters. The human apoA-II gene encodes the second most abundant protein of HDL particles, where it influences plasma levels of free fatty acids (FFA). The human apoA-IV gene encodes a 396 amino acid preprotein, which after proteolytic processing is secreted from the intestine in association with chylomicron particles. ApoA-IV is a potent activator of lecithin-cholesterol acyltransferase (LCAT) in vitro. The human apoA-V gene encodes a 366 amino acid protein that is believed to be an important determinant of plasma triglyceride levels.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Since it works for human sera extract, I was wondering if it would also work for detecting recombinant human ApoA5 in mouse plasma? Also, how much human sera did you load per lane and which secondary did you use?
Asked by: tarapopp
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext.1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-11-09
What blocking solution should I use with apoA-V (1G5G9): sc-32809 monoclonal antibody for Western blot?
Asked by: AbPolly
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here:
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