Apolipoprotein A I/APOA1 Antibody (B-10) recommended for detection of apoA-I of human origin by WB, IP, IF, IHC(P) and ELISA

Apolipoprotein A I/APOA1 Antibody (B-10): sc-376818

Apolipoprotein A I/APOA1 Antibody (B-10) is rated 4.5 out of 5 by 10.
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Datasheets

    • Apolipoprotein A I/APOA1 Antibody (B-10) is a mouse monoclonal IgG2b κ Apolipoprotein A I/APOA1 antibody, cited in 42 publications, provided at 200 µg/ml
    • raised against amino acids 1-267 representing full length apoA-I of human origin
    • Apolipoprotein A I/APOA1 Antibody (B-10) is recommended for detection of apoA-I of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Apolipoprotein A I/APOA1 Antibody (B-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Apolipoprotein A I/APOA1 (B-10): sc-376818.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Apolipoprotein A I/APOA1 Antibody (B-10) for WB and IHC(P) applications. These reagents are now offered in bundles with Apolipoprotein A I/APOA1 Antibody (B-10) (see ordering information below).
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Do you know of any cases, where the antibody was used to bind to the ApoA1-derived membrane scaffold protein?

Asked by: Umbach27
Thank you for your question. We only have data for the detection of apoA-I of human origin in WB, IP, IF, IHC(P) and ELISA applications.
Answered by: Tech Support Europe
Date published: 2021-01-20

Is antigen retrieval required with working with paraffin-embedded sections?

Asked by: AbPolly
Thank you for your question. Yes, we recommend performing antigen retrieval when using this antibody for IHC(P). Our recommended method is citrate buffer, pH 6, and heat. Our full protocol can be found on the website here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 13
Date published: 2017-02-09

Can we purchase following two items from vendors in India right now? are these two items are available in santa cruz right now? sc-376818 and sc-516102

Asked by: saikatm91
Thank you for your question. This primary antibody and mouse binding protein are typically available in stock, however please inquire with our Customer Care Department for the most up to date information about product availability. Both products are currently available to be ordered through one of our authorized distributors in India. A link to search for contact information for our distributors is provided below. https://www.scbt.com/scbt/resources/distributors
Answered by: Technical Support
Date published: 2017-01-10

can sc-7297 is being uesed as secondery antibody for sc-376818

Asked by: saikatm91
Thank you for your question. No, it can not be used as a secondary antibody. sc-7297 is the catalog number for a mouse monoclonal primary antibody against HCAM (DF1485). And sc-376818 is the catalog number for a different mouse monoclonal primary antibody against apoA-I (B-10). A list of recommended secondary antibodies to be used with this primary antibody can be found on the product page. Choose which application you are using the primary antibody in, and a list of products is provided.
Answered by: Technical Support
Date published: 2017-01-05
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Rated 4 out of 5 by from APOA1 This antibody is good,the bands were clear but had some unnecerssary bands
Date published: 2017-08-25
Rated 5 out of 5 by from Clean and good sensitivity Produced a single band at expected MW in HepG2 cell extract and human kidney lysate. No protein detected in HEK293, possibly the protein is not expressed in this cell line?
Date published: 2017-07-24
Rated 5 out of 5 by from Apolipoprotein A1 antibody for human samples I used this antibody for the human samples and used for immunoblotting as well as IHC both i found nice results.
Date published: 2017-03-03
Rated 5 out of 5 by from functional ab ApoE in conditioned media of primary astrocytes from human apoE3/3 and apoE4/4 heterozygous mice was analyzed. Two bands were observed. These 2 bands were also observed when another anti-ApoE antibody (Non-sc) was used
Date published: 2017-02-16
Rated 5 out of 5 by from Good for detecting the human protein A good choice for apoA detection. It provides clear and strong signal in blotting and staining procedures. This product has been published with.
Date published: 2017-01-27
Rated 1 out of 5 by from Could not detect anything on my blot or IP Could not detect anything on my blot or IP.
Date published: 2015-03-27
Rated 5 out of 5 by from Worked well in formalin fixed Worked well in formalin fixed, paraffin-embedded human kidney tissue for IHC. -SCBT QC
Date published: 2015-03-19
Rated 5 out of 5 by from Immunofluorescence staining of formalin Immunofluorescence staining of formalin-fixed HepG2 cells shows golgi apparatus localization. -SCBT QC
Date published: 2015-02-11
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