Anti-API5 Antibody (E-12) is a mouse monoclonal IgG1 (kappa light chain) API5 antibody provided at 200 µg/ml
raised against amino acids 1-300 mapping at the N-terminus of API5 of human origin
Anti-API5 Antibody (E-12) is recommended for detection of API5 of mouse, rat and human origin by WB, IP, IF and ELISA
Anti-API5 Antibody (E-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of API5 (E-12): sc-374528.
m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for API5 Antibody (E-12) for WB applications. These reagents are now offered in bundles with API5 Antibody (E-12) (see ordering information below).
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
API5 Antibody (E-12) is a high quality monoclonal API5 antibody (also designated API5 antibody) suitable for the detection of the API5 protein of mouse, rat and human origin. API5 Antibody (E-12) is available as both the non-conjugated anti-API5 antibody form, as well as multiple conjugated forms of anti-API5 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. API5 (apoptosis inhibitor 5), also known as AAC11 (antiapoptosis clone 11 protein), FIF (fibroblast growth factor 2-interacting factor), MIG8, XAGL or API5L1, is a widely expressed antiapoptotic nuclear protein that is highly conserved from flies to humans. API5 contains a nuclear localization sequence, an LxxLL motif, a leucine zipper domain and a transactivation domain flanked by two acidic domains. API5 forms a nuclear localized complex with FGF-2 and may mediate FGF-2-dependent signaling. API5 is believed to function as a transcription regulator and is able to regulate the synthesis of MMP-2 (matrix metalloproteinase-2). In addition, API5 is known to specifically suppress E2F-dependent apoptosis. It is expressed in a variety of cancer cell lines and its expression is linked to tumor progression and the degree of malignancy.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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