Antipain, Dihydrochloride is a reversible protease inhibitor produced by actinomycetes. Antipain inhibits cathepsin A, B, and D as well as papain, trypsin and trypsin-like serine proteases. Antipain acts by forming a hemiacetal adduct between the aldehyde group of the inhibitor and the active proteinase. Effective at concentrations range from 1 to 100 μM.
Inhibitory Activity ≥30 U/mg (Trypsin, L-BAPA)
Soluble in water (50 mg/ml), DMSO (5 mg/ml), 1-butanol, 1-propanol, ethanol, and methanol. Insoluble in chloroform, benzene, and hexane.
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DamascenoDamasceno, et al. (PubMed ID 25822177) used antipain conjugated to Sepharose to separate endogenous kininogenases from CHO-K1 lysate fraction. -SCBT Publication Review
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