Anti-c-Myc Magnetic Beads are affinity particles for immunoprecipitation of recombinant c-Myc-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators.
Features of Anti-c-Myc Magnetic Beads:
• Specific—highly specific anti-c-Myc monoclonal antibody (clone 9E10) enables high yield and high purity immunoprecipitation.
• Convenient and fast—product instructions provide an easy-to-follow, optimized protocol for immunoprecipitation in approximately one hour.
• Low non-specific binding—stable, pre-blocked beads and specific antibody minimize off-target binding for c-Myc-tag IP or co-IP experiments.
• Versatile—beads are compatible with manual and automated workflows.
The blocked magnetic bead surface is coated with anti-c-Myc antibody, a highly specific mouse IgG1 monoclonal antibody (clone 9E10) that recognizes the c-Myc-epitope tag (EQKLISEEDL) derived from the human c-myc oncogene (p62 c-myc). Anti-c-Myc Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms.
Anti-c-Myc Magnetic Beads are convenient for the immunoprecipitation (IP) of recombinant c-Myc tagged proteins and the co-immunoprecipitation (Co-IP) of their interacting proteins. The beads are incubated with a cell lysate containing c-Myc tagged protein and the fusion protein is captured. The beads are subsequently washed and then the target proteins are eluted using 0.1M glycine (pH 2.0), 50 mM NaOH, or SDS-PAGE sample buffer.