Anti-c-Myc Magnetic Beads For immunoprecipitation of recombinant c-Myc-tagged proteins.

Anti-c-Myc Magnetic Beads

Anti-c-Myc Magnetic Beads is rated 5.0 out of 5 by 1.
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Application: Anti-c-Myc Magnetic Beads is for immunoprecipitation of recombinant c-Myc-tagged proteins
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
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Anti-c-Myc Magnetic Beads are affinity particles for immunoprecipitation of recombinant c-Myc-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators.

Features of Anti-c-Myc Magnetic Beads:
• Specific—highly specific anti-c-Myc monoclonal antibody (clone 9E10) enables high yield and high purity immunoprecipitation.
• Convenient and fast—product instructions provide an easy-to-follow, optimized protocol for immunoprecipitation in approximately one hour.
• Low non-specific binding—stable, pre-blocked beads and specific antibody minimize off-target binding for c-Myc-tag IP or co-IP experiments.
• Versatile—beads are compatible with manual and automated workflows.

The blocked magnetic bead surface is coated with anti-c-Myc antibody, a highly specific mouse IgG1 monoclonal antibody (clone 9E10) that recognizes the c-Myc-epitope tag (EQKLISEEDL) derived from the human c-myc oncogene (p62 c-myc). Anti-c-Myc Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms.

Product Details:
Anti-c-Myc Magnetic Beads are convenient for the immunoprecipitation (IP) of recombinant c-Myc tagged proteins and the co-immunoprecipitation (Co-IP) of their interacting proteins. The beads are incubated with a cell lysate containing c-Myc tagged protein and the fusion protein is captured. The beads are subsequently washed and then the target proteins are eluted using 0.1M glycine (pH 2.0), 50 mM NaOH, or SDS-PAGE sample buffer.

Usage :
•Do not centrifuge, dry or freeze Anti-c-Myc Magnetic Beads, as this can cause the beads to aggregate and lose binding activity. To ensure good dispersal of beads for optimal antibody binding, it is important to include 0.025% to 0.1% non-ionic (e.g., sc-29113, Tween-20 Detergent) or zwitterionic (e.g., sc-29088, CHAPS) detergent in the binding and wash buffers and to mix the beads during incubation.

• For best results, determine optimal conditions for expression of c-Myc-tagged fusion protein before attempting immunoprecipitation.

• To minimize protein degradation, include protease inhibitors (such as: sc-29130, UltraCruz® Protease Inhibitor Cocktail Tablet or sc-29131, UltraCruz® Protease Inhibitor Cocktail Tablet, EDTA-free) when preparing cell lysates.

• Binding capacity and elution recovery will vary depending on the c-Myc-fusion protein and the elution method.

• A low-pH elution may be used for single-use applications. Optimal incubation time for low-pH elution is 5-10 minutes; exceeding 10 minutes may result in nonspecific binding and yield reduction. The c-Myc antibody will not leach from the beads when eluting with the recommended acidic elution buffer (0.1M glycine, pH 2.0).

• Basic elution buffer (e.g., 50mM NaOH) may be used to elute c-Myc-tagged protein; however, the stringency of the buffer will cause the c-Myc antibody to leach from the beads.

• If a gentle elution of c-Myc-tagged protein is desired, a competitive elution can be performed using 0.5mg/mL of c-Myc Peptide.

• Anti-c-Myc Magnetic Beads are compatible with IP and Western blot analysis.

• Do not use cell lysate containing dithiothreitol (DTT). DTT may cause the c-Myc antibody to leach from the beads.
Formulation :
10mg/mL suspension in PBS with 0.05% Tween™-20 Detergent and 0.05% NaN3
Appearance :
Suspension
Physical State :
Liquid
Storage :
Store at 4° C
Density :
2.0 g/mL
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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Certificate of Analysis

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Anti-c-Myc Magnetic Beads  Product Citations

See how others have used Anti-c-Myc Magnetic Beads. Click on the entry to view the PubMed entry .

Citations 1 to 1 of 1 total

PMID: # 20421418  Lau, A. et al. 2010. Mol. Cell. Biol. 30: 3275-85.

Citations 1 to 1 of 1 total

What is the binding capacity of the beads?

Asked by: Anonymous
Thank you for your question. binding capacity is ≥0.07 mg GST c-Myc/ml beads and may vary between different lots.
Answered by: Tech Support Europe
Date published: 2020-12-18

What size are the beads?

Asked by: EddieJames
Thank you for your question. The mean diameter is approximately 1 µm.
Answered by: Tech Service
Date published: 2019-11-19
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Rated 5 out of 5 by from Worked well in ourhands We used the beads to co-IP the mitochondrial proteins after gentle lysis. All controls worked well, we didn't detect any Myc-specific signal in the wash (unbound) fraction after 24 h incubation.
Date published: 2017-08-23
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