Annexin VI Antibody (E-5) recommended for detection of Annexin VI of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Annexin VI Antibody (E-5): sc-271859

Annexin VI Antibody (E-5) is rated 4.9 out of 5 by 8.
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Datasheets

    • Annexin VI Antibody (E-5) is a mouse monoclonal IgG1 κ Annexin VI antibody, cited in 6 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 5-27 at the N-terminus of Annexin VI of human origin
    • Annexin VI Antibody (E-5) is recommended for detection of Annexin VI of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Annexin VI Antibody (E-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Annexin VI (E-5): sc-271859.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for Annexin VI Antibody (E-5) for WB and IHC(P) applications. This reagent is now offered in a bundle with Annexin VI Antibody (E-5) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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I'm interested in testing this (sc-271859 ) antibody on zebrafish and would like to ask if it is possible to get a test sample?

Asked by: Alexandra73
Thank you for your question. Please contact our technical service team to arrange for a free sample of this antibody.
Answered by: Tech Support Europe
Date published: 2022-03-25

What application is the blocking peptide sc-271859 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Ottimo funzionamento!!! L’anticorpo anti-Annexin VI (sc-271859), richiesto con l’Ordine n° 535 dell’11/11/2021 e arrivato il giorno 23/11/2021, è stato testato su un lisato di cellule HeLa, ottenuto tramite omogenizzazione manuale in una soluzione di PBS-0.1% Igepal contenente un cocktail di inibitori di proteasi. Aliquote di lisato da 2 μg, 5 μg, 10 μg e 20 μg di proteine sono state caricate su un gel di poliacrilammide al 10% e sottoposte a Western Blotting. La membrana così ottenuta è stata sottoposta a blocking (in una soluzione al 5% di non-fat dried milk in TBS-0.05% TWEEN 20) e, successivamente, incubata con l’anticorpo primario sopra riportato (1:1000 vol/vol). La membrana è poi stata incubata con un anticorpo secondario anti-mouse (1:1500 vol/vol) coniugato con perossidasi di rafano. I segnali di chemiluminescenza sono stati rivelati tramite il sistema di imaging digitale Amersham Imager 680 (GE Healthcare Life Sciences). Come mostrato nell’immagine di seguito riportata, l’anticorpo produce segnali in corrispondenza del peso molecolare dell’Annessina VI in maniera concentrazione dipendente.
Date published: 2021-11-25
Rated 5 out of 5 by from This antibody is good for WB and IP I got this antibody few month before, it is good for both WB and IP
Date published: 2017-11-20
Rated 5 out of 5 by from works great on western blot and IHC We have used this antibody on human skin tissue. It works great on both western blot and IHC.
Date published: 2017-09-20
Rated 4 out of 5 by from WORKED WELL I received this antibody as a free trial sample and used for WB and Immunoprecipitation. It worked well.
Date published: 2017-09-11
Rated 5 out of 5 by from Distinct band observed via WB using product sc Distinct band observed via WB using product sc-271859 using HeLa whole cell lysate. -SCBT QC
Date published: 2015-04-12
Rated 5 out of 5 by from Good specificity shown by using transfected Good specificity shown by using transfected and non-transfected 293T cells for over-expression of the human Annexin VIII protein via Western Blot analysis. -SCBT QC
Date published: 2015-03-29
Rated 5 out of 5 by from Positive detection of endogenous Annexin Positive detection of endogenous Annexin VIII in JAR cells by WB analysis. -SCBT QC
Date published: 2015-03-21
Rated 5 out of 5 by from Produced excellent immunofluorescence membrane Produced excellent immunofluorescence membrane and cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-02-26
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