Annexin III Antibody (E-11) recommended for detection of Annexin III of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Annexin III Antibody (E-11): sc-390502

Annexin III Antibody (E-11) is rated 4.7 out of 5 by 3.
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Datasheets

    • Annexin III Antibody (E-11) is a mouse monoclonal IgG2a κ Annexin III antibody, cited in 1 publications, provided at 200 µg/ml
    • raised against amino acids 1-50 mapping at the N-terminus of Annexin III of human origin
    • Annexin III Antibody (E-11) is recommended for detection of Annexin III of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Annexin III Antibody (E-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Annexin III (E-11): sc-390502.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Annexin III Antibody (E-11) for WB and IHC(P) applications. These reagents are now offered in bundles with Annexin III Antibody (E-11) (see ordering information below).
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What is the recommended fixation method for immunofluorescence staining with Annexin III (E-11): sc-390502 monoclonal antibody?

Asked by: Professor Griffin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from Large band observed at corresponding molecular Large band observed at corresponding molecular weight for Annexin III observed by WB using human lung tissue extract. -SCBT QC
Date published: 2015-05-17
Rated 5 out of 5 by from Positive detection of endogenous Annexin Positive detection of endogenous Annexin III expression in human placenta tissue extract by WB. -SCBT QC
Date published: 2015-04-02
Rated 5 out of 5 by from Produced great immunofluorescence cytoplasmic Produced great immunofluorescence cytoplasmic and membrane staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-20
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