Annexin-2/ANXA2 Antibody (3D5) recommended for detection of Annexin II of mouse, rat and human origin by WB, IP and IF

Annexin-2/ANXA2 Antibody (3D5): sc-47696

Annexin-2/ANXA2 Antibody (3D5) is rated 5.0 out of 5 by 7.
  • y_2022, m_11, d_30, h_21
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.34
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_7
  • loc_en_US, sid_47696, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 150ms
  • REVIEWS, PRODUCT
loading
5
7
4
0
3
0
2
0
1
0

Datasheets

    • Annexin-2/ANXA2 Antibody (3D5) is a mouse monoclonal IgG2a κ, cited in 16 publications, provided at 200 µg/ml
    • raised against whole native Annexin proteins from human bone tissues
    • Anti-Annexin-2/ANXA2 Antibody (3D5) is recommended for detection of Annexin II of mouse, rat and human origin by WB, IP and IF
    • Anti-Annexin-2/ANXA2 Antibody (3D5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Annexin-2/ANXA2 (3D5): sc-47696.
    • m-IgG Fc BP-HRP is the preferred secondary detection reagent for Annexin-2/ANXA2 Antibody (3D5) for WB applications. This reagent is now offered in a bundle with Annexin-2/ANXA2 Antibody (3D5) (see ordering information below).
loading

What about cross-reactivity with annexinA1 and annexin A3?

Asked by: mtsanna
Thank you for your question. This antibody is specific for Annexin II and should not pick up Annexin 1 and Annexin III. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-11-18

What is the recommended fixation method for immunofluorescence staining with Annexin II (3D5): sc-47696 monoclonal antibody?

Asked by: TinTin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2022, m_11, d_30, h_21CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.34
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_en_US, sid_47696, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 170ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Works Great All of the Santa Cruz Annexin II antibodies have been good experiences for us.
Date published: 2019-02-06
Rated 5 out of 5 by from Specific staining (cell membranes) Specific staining (cell membranes). Excellent antibody for IHC. Reagents for blocking steps & seconary antibody from Dako
Date published: 2017-02-14
Rated 5 out of 5 by from Robust band seen in WB in K Robust band seen in WB in K-562 whole cell lysate. -SCBT QC
Date published: 2015-05-18
Rated 5 out of 5 by from Good specificity in WB in human Annexin II Good specificity in WB in human Annexin II transfected 293 whole cell lysate. -SCBT QC
Date published: 2015-04-28
Rated 5 out of 5 by from Published data for WB and IF in HEK293T cells Published data for WB and IF in HEK293T cells. -SCBT Publication Review
Date published: 2015-04-16
Rated 5 out of 5 by from Strong band in WB in HeLa whole cell lysate Strong band in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-04-08
Rated 5 out of 5 by from Exceptional immunofluorescence membrane staining Exceptional immunofluorescence membrane staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-14
  • y_2022, m_11, d_30, h_21
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.34
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_7
  • loc_en_US, sid_47696, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 22ms
  • REVIEWS, PRODUCT