Annexin I Antibody (74/3) recommended for detection of Annexin I of human origin by WB and IP

Annexin I Antibody (74/3): sc-65872

Annexin I Antibody (74/3) is rated 5.0 out of 5 by 2.
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Datasheets

    • Annexin I Antibody (74/3) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
    • raised against AG1523 fibroblasts of human origin
    • recommended for detection of Annexin I of human origin by WB and IP
    • See Annexin I (EH17a): sc-12740 for Annexin I antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Annexin I Antibody (74/3) for WB applications. These reagents are now offered in bundles with Annexin I Antibody (74/3) (see ordering information below).
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What blocking solution should I use with Annexin I (74/3): sc-65872 monoclonal antibody for Western blot?

Asked by: AbPolly
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from Good specificity shown by using transfected Good specificity shown by using transfected and non-transfected 293T cells for over-expression of the human Annexin I protein via Western Blot analysis. -SCBT QC
Date published: 2015-01-05
Rated 5 out of 5 by from Positive detection of endogenously expressed Positive detection of endogenously expressed Annexin I in ECV304 and K562 cells by WB. -SCBT QC
Date published: 2013-11-01
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