Annexin A9 Antibody (F-9) recommended for detection of Annexin A9 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Annexin A9 Antibody (F-9): sc-374288

Annexin A9 Antibody (F-9) is rated 5.0 out of 5 by 3.
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Datasheets

    • Annexin A9 Antibody (F-9) is a mouse monoclonal IgG1 κ Annexin A9 antibody provided at 200 µg/ml
    • raised against amino acids 186-270 mapping within an internal region of Annexin A9 of human origin
    • Annexin A9 Antibody (F-9) is recommended for detection of Annexin A9 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Annexin A9 Antibody (F-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Annexin A9 (F-9): sc-374288.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Annexin A9 Antibody (F-9) for WB and IHC(P) applications. These reagents are now offered in bundles with Annexin A9 Antibody (F-9) (see ordering information below).
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Can Annexin A9 (F-9): sc-374288 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Cweed
Thank you for your question. Yes, Annexin A9 (F-9): sc-374288 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from Superb immunoperoxidase cytoplasmic and membrane Superb immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human gall bladder tissue. -SCBT QC
Date published: 2015-04-17
Rated 5 out of 5 by from Good specificity shown by using transfected Good specificity shown by using transfected and non-transfected 293T cells for over-expression of the mouse Annexin A9 protein via Western Blot analysis. -SCBT QC
Date published: 2015-04-05
Rated 5 out of 5 by from Positive detection of endogenous Annexin Positive detection of endogenous Annexin A9 protein in BJ whole cell lysate observed by WB. -SCBT QC
Date published: 2015-02-27
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