Angptl4 Antibody (C-7) recommended for detection of Angptl4 of human origin by WB, IP, IF and ELISA

Angptl4 Antibody (C-7): sc-373762

Angptl4 Antibody (C-7) is rated 4.8 out of 5 by 4.
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Datasheets

    • Angptl4 Antibody (C-7) is a mouse monoclonal IgG2b κ, cited in 2 publications, provided at 200 µg/ml
    • raised against amino acids 51-250 mapping near the N-terminus of Angptl4 of human origin
    • Angptl4 Antibody (C-7) is recommended for detection of Angptl4 of human origin by WB, IP, IF and ELISA
    • Anti-Angptl4 Antibody (C-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Angptl4 (C-7): sc-373762.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Angptl4 Antibody (C-7) for WB applications. These reagents are now offered in bundles with Angptl4 Antibody (C-7) (see ordering information below).
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What blocking solution should I use with Angptl4 (C-7): sc-373762 monoclonal antibody for Western blot?

Asked by: Cweed
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-04-13
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Rated 4 out of 5 by from IF Works somewhat in mouse lung tissues for IF. I would give it a try.
Date published: 2017-12-22
Rated 5 out of 5 by from Great antibody Wonderful antibody for Western blot, specific and reliable
Date published: 2017-12-19
Rated 5 out of 5 by from Strong antibody for WB Good monoclonal antibody for Western blot, specific and reliable.
Date published: 2017-02-03
Rated 5 out of 5 by from Produced nice Western blot data of Angptl4 Produced nice Western blot data of Angptl4 expression in non-transfected and human Angptl4 transfected whole cell lysates. -SCBT QC
Date published: 2014-09-30
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