Angptl2 Antibody (H-2) is a mouse monoclonal IgG1 κ Angptl2 antibody, cited in 1 publications, provided at 200 µg/ml
raised against amino acids 158-254 mapping within an internal region of Angptl2 of human origin
Angptl2 Antibody (H-2) is recommended for detection of Angptl2 of mouse, rat and human origin by WB, IP, IF and ELISA
Anti-Angptl2 Antibody (H-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Angptl2 (H-2): sc-393747.
m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Angptl2 Antibody (H-2) for WB applications. These reagents are now offered in bundles with Angptl2 Antibody (H-2) (see ordering information below).
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Angptl2 Antibody (H-2) is an IgG1 κ mouse monoclonal Angptl2 antibody (also designated Angptl2 antibody) suitable for the detection of the Angptl2 protein of mouse, rat and human origin by WB, IP, IF and ELISA. Angptl2 Antibody (H-2) is available as both the non-conjugated anti-Angptl2 antibody form, as well as multiple conjugated forms of anti-Angptl2 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Angptl2 (Angiopoietin-like 2), also known as angiopoietin-related protein 2 (ARP2), is a member of the fibrinogen superfamily. It is expressed in adult heart, stomach, spleen and small intestine tissues. Angptl2 consists of an N-terminus with a conserved coiled-coil domain, two glycosylation sites and a C-terminus with a conserved fibrinogen-like domain. It is a secreted protein and shares 59% sequence identity with Angptl1. In endothelial cells, Angptl2 does not function as a growth factor, instead it functions to induce sprouting and regulate angiogenesis. This activity is thought to be exerted via the interaction of Angptl2 with an endotheilial cell receptor and may involve paracrine and autocrine signalling. The loss of Angptl2 results in impaired vascular development. In addition, upregulation of Angptl2 may play a significant role in the pathogenesis of diabetic glomerulopathy and tumor growth and survival.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz® conjugates which eliminates the need for a secondary antibody.
Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Angptl2 Antibody (H-2) has 1 citations in a variety of scientific publications.
Primary antibodies like Anti-Angptl2 Antibody (H-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.
Angptl2 Antibody (H-2) Specifications
Alternate Product Name
Product Clone Name
IgG1 kappa light chain
mouse, rat, human
Additional Mammalian Reactivity
Additional Non-Mammalian Reactivity
WB, IP, IF, ELISA
Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin
1 citations in various scientific publications
Recommended Secondary Detection Reagents
Mouse IgG Fc Binding Proteins
m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
Primary antibodies for non-mammalian targets are useful in detecting proteins of plant, viral, bacterial, avian, fish, yeast and invertebrates. Some examples include Drosophila, zebrafish, Xenopus, Arabidopsis, C. elegans, Clostridium, Campylobacter, Heliobacter pylori, Hepatitis, HIV, HPV, HSV, Influenza, Klebsiella, M. tuberculosis, Plasmodium, Pseudomonas, RSV, Saccharomyces cerevisiae, Salmonella, Schistosoma, Shigella and Staphylococcus.
Santa Cruz Biotechnology monoclonal antibodies can be used in assays including flow cytometry, immunoprecipitation, Western blot analysis, and immunostaining. Anti-Angptl2 Antibody (H-2) is recommended for use in the following applications: WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), ELISA (Enzyme-Linked Immunosorbent Assay).
Mouse secondary antibodies, control sera and control immunoglobulins are also offered to be used in combination with our primary monoclonal antibodies.
Tissue extracts, nuclear extracts and whole cell lysates are used with Santa Cruz Biotechnology monoclonal antibodies for western blotting or immunoprecipitation positive controls. Nuclear extracts are ideal for western blot analysis or gel shift assays positive controls. Phospho-enriched whole cell lysates and tissue extracts are available for phospho-specific antibody positive controls.
Monoclonal secondary antibodies are offered in various conjugated forms, including: Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), and TRITC (tetramethyl rhodamine isothiocyanate) (R), for research applications like Flow Cytometry (FCM), Immunofluorescence (IF), Immunohistochemistry (IHC) staining and Western Blotting (WB). Not intended for diagnostic or therapeutic use.
Affinity purified immunoglobulins (IgGs)—conjugated and unconjugated—are available as negative controls for flow cytometry (FCM), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP) and Western Blotting (WB) applications.
Normal sera for immunohistochemistry (IHC) applications are available as neat serum for blocking reagents. The serum which is used should match the species of the secondary antibody.
I need to use an antibody to detect the protein in samples of equine origin. Do you know if sc-393747: Angptl2 (H-2) mouse monoclonal antibody will work?
Asked by: Trav11
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Rated 5 out of
good antibodyI applied the free Angptl2 antibody, used in rat ovarian tissue for the expression of Angptl2, diluted in 1:500 with 5% BSA.
Date published: 2017-09-18
Rated 4 out of
Works good in WBGood specificity in WB of mouse tissues with 1:500.
Date published: 2017-06-27
Rated 5 out of
Satisfactory Western blot data of Angptl2Satisfactory Western blot data of Angptl2 expression in non-transfected and human Angptl2 transfected 293T whole cell lysates. -SCBT QC
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