Anti-Angptl1 Antibody (A-5) recommended for detection of Angptl1 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-Angptl1 Antibody (A-5): sc-365146

Anti-Angptl1 Antibody (A-5) is rated 5.0 out of 5 by 2.
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Datasheets

    • Angptl1 Antibody (A-5) is a mouse monoclonal IgG2a κ, cited in 1 publications, provided at 200 µg/ml
    • raised against amino acids 156-315 mapping within an internal region of Angptl1 of human origin
    • recommended for detection of Angptl1 of mouse, rat and human origin by WB, IP, IF and ELISA
    • m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Angptl1 Antibody (A-5) for WB applications. These reagents are now offered in bundles with Angptl1 Antibody (A-5) (see ordering information below).
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What is the recommended fixation method for immunofluorescence staining with Angptl1 (A-5): sc-365146 monoclonal antibody?

Asked by: Germaine
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Beautiful immunofluorescence cytoplasmic Beautiful immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-12
Rated 5 out of 5 by from Positive results in Western blot analysis Positive results in Western blot analysis of Angptl1 expression in Jurkat whole cell lysate. -SCBT QC
Date published: 2015-02-17
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