Anti-ANG I Antibody (C-1) recommended for detection of precursor and mature ANG I of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-ANG I Antibody (C-1): sc-74528

Anti-ANG I Antibody (C-1) is rated 4.9 out of 5 by 11.
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Datasheets

    • Anti-ANG I Antibody (C-1) is a mouse monoclonal IgG2a κ ANG I antibody, cited in 16 publications, provided at 200 µg/ml
    • raised against amino acids 25-147 of ANG I of human origin
    • Anti-ANG I Antibody (C-1) is recommended for detection of precursor and mature ANG I of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-ANG I Antibody (C-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ANG I (C-1): sc-74528.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for ANG I Antibody (C-1) for WB applications. These reagents are now offered in bundles with ANG I Antibody (C-1) (see ordering information below).
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What is the best condition to use this antibody for ANG IP in A549 cells? Thanks!

Asked by: Xiaoyong
Thank you for your question. We do not have any data using this antibody in A549 cells. Please refer to our website for the most up to date information availalbe for this antibody. Please contact our Technical Service Department if you would like further support.
Answered by: Technical Support
Date published: 2019-08-17

Can ANG I (C-1): sc-74528 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Randy McDonald
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, ANG I (C-1): sc-74528 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from ANG I Works perfect in Western Blot. Used in 5% milk powder.
Date published: 2017-10-10
Rated 5 out of 5 by from Geeignet für Western Blot An humanen Fibroblasten ausgestestet, funktioniert sehr gut.
Date published: 2017-09-26
Rated 5 out of 5 by from 抗体的特异性很好,试验进行的很顺利 I buy ANG1 antibody was 2 month ago,and this antibody was very useful
Date published: 2017-09-12
Rated 5 out of 5 by from Funktioniert sehr gut im Western Blot Ich habe eine Probe von SCBT getestet und bin mit dem Ergebnis im WB sehr zufrieden.
Date published: 2017-09-07
Rated 5 out of 5 by from suitable for WB This antibody suitable for ANG I analysis,the antibody is outstanding quality for WB analyisis.Very clear band seen at 17kda, no background.
Date published: 2017-08-26
Rated 5 out of 5 by from performs very well This antibody performs very well and show high reproducibility for different samples, with excellent experimental results at 1:500,got strong signal.
Date published: 2017-07-22
Rated 4 out of 5 by from works good in IHC it works good on western blot using human skin tissue
Date published: 2017-07-05
Rated 5 out of 5 by from signal is wonderfu I use ANG I at a dilution 1:500 in western blot , 4℃ over night , signal is wonderful.
Date published: 2017-06-01
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