Anti-Amphiphysin II Antibody (D-3) recommended for detection of all Amphiphysin II isoforms of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Amphiphysin II Antibody (D-3): sc-74486

Anti-Amphiphysin II Antibody (D-3) is rated 5.0 out of 5 by 3.
  • y_2021, m_10, d_18, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_3
  • loc_en_US, sid_74486, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 113ms
  • REVIEWS, PRODUCT
loading
5
3
4
0
3
0
2
0
1
0

Datasheets

    • Amphiphysin II Antibody (D-3) is a mouse monoclonal IgG2a κ (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 421-520 mapping within an internal region of Amphiphysin II of human origin
    • recommended for detection of all Amphiphysin II isoforms of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See Amphiphysin II (2F11): sc-23918 for Amphiphysin II antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Amphiphysin II Antibody (D-3) for WB and IHC(P) applications. These reagents are now offered in bundles with Amphiphysin II Antibody (D-3) (see ordering information below).
loading

I am using Amphiphysin II (D-3): sc-74486 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2021, m_10, d_18, h_18CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_74486, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 131ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Superb immunoperoxidase cytoplasmic staining Superb immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human spleen tissue. -SCBT QC
Date published: 2015-04-17
Rated 5 out of 5 by from Positive results in western blot analysis Positive results in western blot analysis of Amphiphysin II expression in HeLa nuclear extract, Jurkat and SJRH30 whole cell lysates and rat skeletal muscle tissue extract. -SCBT QC
Date published: 2015-04-06
Rated 5 out of 5 by from Clean bands in western blot using truncated Clean bands in western blot using truncated human Amphiphysin II transfected 293T and HeLa whole cell lysates. -SCBT QC
Date published: 2015-03-27
  • y_2021, m_10, d_18, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_3
  • loc_en_US, sid_74486, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 14ms
  • REVIEWS, PRODUCT