Anti-Amphiphysin II Antibody (2F11) recommended for detection of all Amphiphysin II isoforms of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-Amphiphysin II Antibody (2F11): sc-23918

Anti-Amphiphysin II Antibody (2F11) is rated 5.0 out of 5 by 7.
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Datasheets

    • Anti-Amphiphysin II Antibody (2F11) is a mouse monoclonal IgG1 κ Amphiphysin II antibody, cited in 18 publications, provided at 200 µg/ml
    • raised against a chimeric protein corresponding to Amphiphysin II BAR domain with an epitope mapping to amino acids 179-207 of human origin
    • Anti-Amphiphysin II Antibody (2F11) is recommended for detection of all Amphiphysin II isoforms of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • Anti-Amphiphysin II Antibody (2F11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Amphiphysin II (2F11): sc-23918.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Amphiphysin II Antibody (2F11) for WB and IHC(P) applications. These reagents are now offered in bundles with Amphiphysin II Antibody (2F11) (see ordering information below).
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is SC23918 affinity purified, if not what is the purity level of this antibody Thank you

Asked by: Kaouther
Thank you for your question. This antibody is not affinity purified. It is provided as a purified mouse monoclonal IgG1. The exact purity level of this antibody has not been determined.
Answered by: Technical Support
Date published: 2020-03-09

Hi, I need to ask how much volume have free samples of antibodies

Asked by: Carolinadiaz
Thank you for your question. Each sample has a concentration of 10 ug of antibody in 50 uL of solution.
Answered by: Technical Support
Date published: 2019-07-18

What is the composition of buffer used for lysis of cells before proceeding for WB?

Asked by: Ipsita
Thank you for your question. We recommend using our RIPA Lysis Buffer System: sc-24948. A link to the product page on our website is below, which shows the composition of the buffer. I have also included a link to our recommended protocol for WB, which includes our complete recommendation for sample preparation of cell lysates prior to WB. https://www.scbt.com/scbt/product/ripa-lysis-buffer-system https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-07-25

Can you recommend a positive control to use with Amphiphysin II (2F11): sc-23918 monoclonal antibody for Western blot?

Asked by: jenni
We recommend C2C12 (sc-364188), Sol8 (sc-2249) or L8 (sc-3807) whole cell lysates to use as positive controls as we have Western blot data showing specific detection of Amphiphysin II in these cell lines.
Answered by: Technical Support 7
Date published: 2017-07-25
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Rated 5 out of 5 by from Great antibody! 1:500 diluted for wb of rat heart protein ,the band is robust!
Date published: 2017-05-21
Rated 5 out of 5 by from Solid antibody Good data in Western blot (WB) and IHC(P) with Amphiphysin II (2F11).
Date published: 2017-01-14
Rated 5 out of 5 by from Strong bands in western blot analysis of Strong bands in western blot analysis of Amphiphysin II expression in C2C12, Sol8 and L8 whole cell lysates. -SCBT QC
Date published: 2015-04-08
Rated 5 out of 5 by from Robust signal in western blot analysis of Robust signal in western blot analysis of Amphiphysin II expression in L8 whole cell lysate. -SCBT QC
Date published: 2015-03-15
Rated 5 out of 5 by from Beautiful immunoperoxidase cytoplasmic staining Beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human skeletal muscle tissue. -SCBT QC
Date published: 2015-02-07
Rated 5 out of 5 by from Published western blot data using LNCaP and Published western blot data using LNCaP and SAOS2 cell lysates. -SCBT Publication Review
Date published: 2015-02-02
Rated 5 out of 5 by from ICC and WB with sheep ICC and WB with sheep, rat and ferret cardiac tissue extracts. -SCBT Publication Review
Date published: 2015-01-27
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