Anti-Amphiphysin II Antibody (2F11) is a mouse monoclonal IgG1 κ Amphiphysin II antibody, cited in 18 publications, provided at 200 µg/ml
raised against a chimeric protein corresponding to Amphiphysin II BAR domain with an epitope mapping to amino acids 179-207 of human origin
Anti-Amphiphysin II Antibody (2F11) is recommended for detection of all Amphiphysin II isoforms of mouse, rat and human origin by WB, IP, IF and IHC(P)
Anti-Amphiphysin II Antibody (2F11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Amphiphysin II (2F11): sc-23918.
m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Amphiphysin II Antibody (2F11) for WB and IHC(P) applications. These reagents are now offered in bundles with Amphiphysin II Antibody (2F11) (see ordering information below).
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possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Amphiphysin II Antibody (2F11) is a high quality monoclonal Amphiphysin II antibody (also designated BIN1 antibody, Bridging Integrator 1 antibody, Myc Box-Dependent-Interacting Protein 1 antibody, Amphiphysin-Like Protein antibody, AMPH2 antibody, AMPHL antibody, SH3P9 antibody, Box Dependant MYC Interacting Protein 1 antibody, or CNM2 antibody) suitable for the detection of the Amphiphysin II protein of mouse, rat and human origin. Amphiphysin II Antibody (2F11) is available as both the non-conjugated anti-Amphiphysin II antibody form, as well as multiple conjugated forms of anti-Amphiphysin II antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Amphiphysin is a brain-enriched protein that exhibits N-terminal lipid interaction and functions as a dimer. Amphiphysin contains a membrane bending BAR domain, a middle Clathrin and adaptor binding domain, and a C-terminal SH3 domain. In the brain, Amphiphysin I and II form heterodimers that bind to the Clathrin associated GTPase Dynamin via their SH3 domains. This association is essential for synaptic vesicle recycling in neurons, as it precedes the binding of Dynamin to the Clathrin-coated pits and the subsequent vesicle budding. In other tissues, Amphiphysin may play a key role in other membrane bending and curvature stabilization events. The mammalian Amphiphysins, Amphiphysin I and Amphiphysin II, have similar overall structure. a ubiquitous splice form of Amphiphysin II that does not contain Clathrin or adaptor interactions is highly expressed in muscle tissue and is involved in the formation and stabilization of the T tubule network.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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