Ammonium acetate solution, 5MAn important reagent for studying molecular biology

Ammonium acetate solution, 5M (CAS 631-61-8)

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Synonym: Azanium Acetate; Acetic acid, ammonium salt
Application: An important reagent for studying molecular biology
CAS Number: 631-61-8
Molecular Weight: 77.08
Molecular Formula: C2H7NO2
* Refer to Certificate of Analysis for lot specific data (including water content).
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Ammonium acetate solution, 5M is an important reagent for studying molecular biology, biological buffers, reagents and DNA and RNA purification. It is a popular buffer for mobile phases for HPLC with ESLD detection, for ESI mass spectrometry of proteins and other molecules, and has been used to replace cell buffers with non-volitile salts. Ammonium acetate is also used in protein studies and protein preparation. It can be used in the protein purification steps of dialysis to remove contaminants through diffusion and, when combined with distilled water, as a protein precipitating agent. In organic chemistry, ammonium acetate solution is useful as a catalyst in the Knoevenagel condensation and as a source of ammonia in the Borch reaction. Additionally, it is occasionally used commercially as a biodegradable de-icing agent and as an additive in food as an acidity regulator.


1. Green, D.P. 1991. J. Cell. Sci. 100: 761-769. PMID: 1814930
2. Prusov, A.N., et al. 1993. Biochem. Biophys. Res. Commun. 193: 591-598. PMID: 8512560
3. Wiedmer, S.K., et al. 1998. Electrophoresis. 19: 1711-1718. PMID: 9719550
4. Desiderio, C., et al. 2001. Electrophoresis. 22: 535-543. PMID: 11258766
5. Berman, E.S., et al. 2008. J. Am. Soc. Mass Spectrom. 19: 1230-1236. PMID: 18565760

Formulation :
5 M Ammonium acetate solution in water
Physical State :
Solubility :
Soluble in alcohol (freely), acetone (slightly), water (148 g/100ml) at 4° C, and water (7.89 g/100ml) at 15° C.
pH :
6.2-7.5 (25° C, 1 M in H2O)
Storage :
Store at room temperature
Density :
1.07 g/mL at 20° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
PubChem CID :
Merck Index :
14: 495
MDL Number :
EC Number :
Beilstein Registry :

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Certificate of Analysis

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is this Hplc grade?

Asked by: Ruchi008
Thank you for your question. This is research grade. Please refer to a recent Certificate of Analysis (CoA) for the specifications to see if it would be suitable for your application. If you do not have a specific lot number, please contact our Technical Service Department to request a CoA from a recent or current lot.
Answered by: Technical Support
Date published: 2019-09-11
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