Anti-Ameloblastin Antibody (H-2) recommended for detection of Ameloblastin of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Ameloblastin Antibody (H-2): sc-271012

Anti-Ameloblastin Antibody (H-2) is rated 3.5 out of 5 by 4.
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Datasheets

    • Anti-Ameloblastin Antibody (H-2) is a mouse monoclonal IgG1 κ Ameloblastin antibody, cited in 2 publications, provided at 200 µg/ml
    • raised against amino acids 148-447 mapping at the C-terminus of Ameloblastin of human origin
    • Ameloblastin Antibody (H-2) is recommended for detection of Ameloblastin of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Ameloblastin Antibody (H-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Ameloblastin (H-2): sc-271012.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Ameloblastin Antibody (H-2) for WB and IHC(P) applications. These reagents are now offered in bundles with Ameloblastin Antibody (H-2) (see ordering information below).
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Can Ameloblastin (H-2): sc-271012 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Randy McDonald
Thank you for your question. Yes, Ameloblastin (H-2): sc-271012 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 3 out of 5 by from Works for Westerns not for IHC in mouse tissue This antibody worked for recombinant murine and native porcine Ambn Western blots but did not work in IHC staining of mouse sections. Previous M300 ameloblastin antibody (rabbit polyclonal) used to work very well (1:500 dilution for IHC) and N18 ameloblastin ab (goat polyclonal) worked at higher concentrations (1:100). I have tried both concentrations for H2 and it did not work.
Date published: 2018-11-12
Rated 1 out of 5 by from Doesn't work in rat and mouse ameloblast SC's previous polyclonal AMBN antibody worked great for me, however this new mouse mono Ab doesn't seem to be equivalent. It doesn't work in my immunofluorescent staining of rat and mouse ameloblasts (paraffin embeding and enzyme/citrate heat retrieval).
Date published: 2017-07-05
Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmic Produced beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-02-07
Rated 5 out of 5 by from Nice Western Blot data of human recombinant Nice Western Blot data of human recombinant Ameloblastin fusion protein. -SCBT QC
Date published: 2014-08-04
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