Anti-alpha Tubulin Antibody (TU-01) recommended for detection of the N-terminal structural domain (amino acids 65-97) of α Tubulin of mouse, rat, human and porcine origin by WB, IP and IF

Anti-α Tubulin Antibody (TU-01): sc-51500

Anti-α Tubulin Antibody (TU-01) is rated 4.0 out of 5 by 2.
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Datasheets

    • α Tubulin Antibody (TU-01) is a mouse monoclonal IgG1, cited in 3 publications, provided at 100 µg/ml
    • raised against fraction of tubulin purified from porcine brain
    • recommended for detection of the N-terminal structural domain (amino acids 65-97) of α Tubulin of mouse, rat, human and porcine origin by WB, IP and IF
    • See α Tubulin (TU-02): sc-8035 for α Tubulin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

How can I see on your website, wether an antibody is produced by directly immunizing animals with synthetic peptides or if it is produced by hybridoma cell cultures? And how is it specifically for "α Tubulin Antikörper (TU-01): sc-51500"?

Asked by: Toxines
Thank you for your question.This monoclonal antibody Tubulin (TU-01): sc-51500 was derived from hybridoma cell culture.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-30

I am using α Tubulin (TU-01): sc-51500 for immunofluorescence, what dilution of this antibody should I use?

Asked by: jerojero
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 3 out of 5 by from Faint WB expression in PC Faint WB expression in PC-12 and K-562 whole cell lysates. -SCBT QC
Date published: 2015-02-12
Rated 5 out of 5 by from Good cytoskeletal staining observed in HeLa cells Good cytoskeletal staining observed in HeLa cells. -SCBT QC
Date published: 2014-10-16
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