Anti-alpha Tubulin Antibody (YOL1/34) recommended for detection of α Tubulin of mouse, rat, human and yeast origin by WB, IP, IF, IHC(P) and ELISA

Anti-α Tubulin Antibody (YOL1/34): sc-53030

Anti-α Tubulin Antibody (YOL1/34) is rated 4.9 out of 5 by 7.
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Datasheets

    • α Tubulin Antibody (YOL1/34) is a rat monoclonal IgG2a, cited in 57 publications, provided at 200 µg/ml
    • raised against full length purified alpha tubulin of Saccharomyces cerevisiae origin
    • alpha Tubulin Antibody (YOL1/34) is recommended for detection of α Tubulin of mouse, rat, human and yeast origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-alpha Tubulin Antibody (YOL1/34) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of α Tubulin (YOL1/34): sc-53030.

Salve a tutti, mi consigliereste un secondario compatibile con questo anticorpo?

Asked by: Sss89
Grazie per la sua domanda. Al momento non offriamo anticorpi secondari o proteine di legame specifici contro gli anticorpi primari di ratto. Per ulteriori informazioni, contattare il nostro servizio tecnico. "What secondary antibody is compatible with this antibody?" Thank you for your question. Unfortunately, we currently do not offer any secondary antibodies or binding proteins specific against rat primary antibodies. Please contact our technical service if you require additional information.
Answered by: Tech Support Europe
Date published: 2019-07-23

Hi, I would like to request a 10 microgram sample of α Tubulin (YOL1/34): sc-53030 for use with western blots of yeast extracts. Thanks! Sincerely, Telsa

Asked by: Telsa
For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department to request a sample, e-mail <[email protected]>.
Answered by: Technical Support 15
Date published: 2017-12-15

Where does the epitope of YOL1/34 antibody bind on the tubulin? Does it bind to the folded or unfolded protein?  Thanks

Asked by: Emmanuel Nsamba
Thank you for your question. This rat monoclonal antibody, α Tubulin (YOL1/34): sc-53030, is raised against full length purified alpha tubulin of Saccharomyces cerevisiae origin. The exact epitope recognized by the antibody has not yet been determined. We have data using the antibody for detection of the denatured (Western blot) protein, as well as the native protein (IF and IHC(P)).
Answered by: Technical Support
Date published: 2017-11-10

Can α Tubulin Antibody (YOL1/34): sc-53030 be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, α Tubulin Antibody (YOL1/34): sc-53030 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 14
Date published: 2017-01-09
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Rated 5 out of 5 by from anti-α-tubulin Excellent monoclonal antibody for the detection of α-tubulin of yeast and human origin. Recommended dilution: 1/1000
Date published: 2019-02-11
Rated 5 out of 5 by from Great for cell homogenates. It did not work for whole tissue homogentes, but it had a great signal for whole cell homogenates.
Date published: 2018-01-31
Rated 5 out of 5 by from Great Antibody for Staining and IF This antibody works very well for a variety of applications including WB, IF, and even IHC. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, yeast, and human samples.
Date published: 2016-12-28
Rated 5 out of 5 by from It seems to work well so far in WB It seems to work well so far in WB.
Date published: 2015-09-16
Rated 4 out of 5 by from Used on human samples in WB Used on human samples in WB, saw band of interest.
Date published: 2015-09-14
Rated 5 out of 5 by from Beautiful IF data Beautiful IF data, specific staining of cytoplasm.
Date published: 2015-03-20
Rated 5 out of 5 by from Works great for Western blot in NIH/3T3 Works great for Western blot in NIH/3T3, HeLa, K-562 and PC-12 whole cell lysate. -SCBT QC
Date published: 2014-07-19
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