Anti-alpha Tubulin Antibody (YL1/2) recommended for detection of α Tubulin of mouse, rat, human and yeast origin by WB, IP, IF, IHC(P) and ELISA

Anti-α Tubulin Antibody (YL1/2): sc-53029

Anti-α Tubulin Antibody (YL1/2) is rated 4.6 out of 5 by 10.
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Datasheets

    • α Tubulin Antibody (YL1/2) is a rat monoclonal IgG2a, cited in 64 publications, provided at 200 µg/ml
    • raised against full length purified α Tubulin of Saccharomyces cerevisiae origin
    • alpha Tubulin Antibody (YL1/2) is recommended for detection of α Tubulin of mouse, rat, human and yeast origin by WB, IP, IF, IHC(P) and ELISA; also recommended for detection of the tyrosinated form of α Tubulin
    • Anti-alpha Tubulin Antibody (YL1/2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of α Tubulin (YL1/2): sc-53029.

I have a question concering α Tubulin Antibody (YL1/2): sc-53029. I would like to know whether it reacts with α Tubulin in Drosophila melanogaster in case of immunostaining and gives positive result?

Asked by: Amid
Thank you for your question. We do not have any information on whether this works on Drosophila melanogaster samples (we do know it works on mouse, rat, human, yeast and dog, so it is possible it works fine). Unfortunately we do not have a sample of this antibody, but we do offer a sample of another alpha tubulin antibody, sc-8035. If you would like to try a sample of that, please contact our European Technical Service team, they would be happy to help you! You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-06-26

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 2
Date published: 2017-01-10
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Rated 4 out of 5 by from Specific signal by western blot We used this antibody on human cultured tenocytes and we obtained a clear band at the expected molecular weight
Date published: 2020-10-12
Rated 5 out of 5 by from Works well in mouse keratinocytes! Our lab uses alpha Tubulin antibody in mouse keratinocytes. It works well when fixed using Methanol at 1:100. We use this antibody often and it looks great!
Date published: 2020-10-01
Rated 5 out of 5 by from Specific bands Specific bands at 55kda, 1:800 used for mouse spleen tissue ,I got ideal result always use this antibody.
Date published: 2017-07-29
Rated 5 out of 5 by from Western blot Western blot using mice brain tissue (60ug) at 1:500 dilution over night incubation for 4℃,nice bands seen.
Date published: 2017-05-09
Rated 5 out of 5 by from Outstanding for all applications Take a look at WB,IF and IHC results; great data!!
Date published: 2017-01-11
Rated 5 out of 5 by from Reliable Antibody, Great for IHC This antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC. Clear signal with little to no background. Beautiful tissue staining. Works well in a variety of species including mouse, rat, yeast, and human samples.
Date published: 2017-01-04
Rated 5 out of 5 by from immunofluorecence in cultured cells works immunofluorecence in cultured cells works really well use at 1:100 in Methanol fix. Also works well in Tissue such as mouse skin and intestinal epithelium.
Date published: 2016-07-27
Rated 5 out of 5 by from The antibody works well for both WB and ICC The antibody works well for both WB and ICC.
Date published: 2015-10-02
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