Anti-alpha Tubulin Antibody (DM1A) recommended for detection of α Tubulin of mouse, rat, human and avian origin by WB, IP, IF and IHC(P)

Anti-α Tubulin Antibody (DM1A): sc-32293

Anti-α Tubulin Antibody (DM1A) is rated 4.8 out of 5 by 21.
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Datasheets

    • α Tubulin Antibody (DM1A) is a mouse monoclonal IgG1 κ, cited in 486 publications, provided at 200 µg/ml
    • raised against native chick brain microtubules
    • Anti-alpha Tubulin Antibody (DM1A) is recommended for detection of α Tubulin of mouse, rat, human and avian origin by WB, IP, IF and IHC(P)
    • Anti-alpha Tubulin Antibody (DM1A) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of α Tubulin (DM1A): sc-32293.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP) are the preferred secondary detection reagents for α Tubulin Antibody (DM1A) for WB and IHC(P) applications. These reagents are now offered in bundles with α Tubulin Antibody (DM1A) (see ordering information below).

Hi, I cannot find on this page an amount of the α Tubulin Antibody (DM1A) sc-32293? It says 200ug/ml, but does not say the volume. Is this antibody genetically identical to T6199 SIGMA? Is it available in -20C buffer?

Asked by: V_M_
Thank you for your question. This antibody is provided as 200 ug IgG in 1 ml. Yes, this clone DM1A is commercially available from other suppliers. Please contact our Special Orders Department to inquire about alternate formulations suitable for -20C storage.
Answered by: Technical Support
Date published: 2019-05-25

How can this mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jerojero
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody.
Answered by: Tech Service 3
Date published: 2016-12-16

I need to use an antibody to detect the protein in samples of avian origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 3
Date published: 2016-12-16
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Rated 5 out of 5 by from Fantastic antibody for western blot Same as that from Sigma or Invitrogen, diluted 1:2000 and can be reused multiple times
Date published: 2021-03-31
Rated 5 out of 5 by from Excellent for WB and immunofluorescence In our hands, we need to fix with PFA 4% and permeabilize with saponin, not Triton, the latter extracts the epitope. But the antibody is clean and very good.
Date published: 2020-11-20
Rated 5 out of 5 by from One of the best antibodies for a-tubulin DM1a is the best alpha-tubulin antibody ever made!. Works quite well in WB for mammalian cell extracts at 1/2000 dilution. Also works in IF detection both Methanol and Formaldehide fixation.
Date published: 2019-01-18
Rated 5 out of 5 by from xx Optimal antibody to be used as a loading control (housekeeping) control in human chronic myeloid leukemia cells as well as many other cells from huma tissues
Date published: 2018-10-16
Rated 5 out of 5 by from good antibody I tried it for IF. Good antibody that gives good signals!
Date published: 2018-06-18
Rated 5 out of 5 by from Adequado para IF en plantas Hemos probado en nuestro laboratorio este anticuerpo con cultivo celular de A. thaliana y hemos obtenido muy buenos resultados. Antes utilizamos anticuerpo de otra marca con precios mas altos pero los resultados con este son iguales.
Date published: 2018-02-22
Rated 5 out of 5 by from very specific High specificity, recommended purchase the monoclonal antibody
Date published: 2017-12-14
Rated 5 out of 5 by from Works with Wes Antibody was specific, but high protein and antibody concentrations were need to get a good signal on the Wes. We used 0.5 mg/ml of protein and Ab at 1:50.
Date published: 2017-09-27
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