Anti-alpha Tubulin Antibody (B-7) recommended for detection of α Tubulin of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA

Anti-α Tubulin Antibody (B-7): sc-5286

Anti-α Tubulin Antibody (B-7) is rated 4.7 out of 5 by 48.
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Datasheets

    • α Tubulin Antibody (B-7) is a mouse monoclonal IgG2a κ, cited in 1,310 publications, provided at 200 µg/ml
    • raised against amino acids 149-448 of α Tubulin of human origin
    • Anti-alpha Tubulin Antibody (B-7) is recommended for detection of α Tubulin of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA; also reactive with additional species, including and canine, bovine and porcine
    • Anti-alpha Tubulin Antibody (B-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to TRITC, PerCP, PerCP-Cy5.5 or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of α Tubulin (B-7): sc-5286.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for α Tubulin Antibody (B-7) for WB and IHC(P) applications. These reagents are now offered in bundles with α Tubulin Antibody (B-7) (see ordering information below).
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I get two bands on my Western Blot (tissue lysate), one at about 56 kDa and another one at approximately 70 kDa. The upper one is much more equal and seems to be a better loading control. Which one should I use for Quantification?

Asked by: Ann22
Thank you for your question. Please contact our technical service directly for assistance.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Anna99
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available conjugated binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 5
Date published: 2016-12-16

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: Anna99
Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal alpha-Tubulin antibody is currently available conjugated to either phycoerythrin (sc-5286 PE), fluoroscein (sc-5286 FITC), Alexa Fluor® 488 (sc-5286 AF488) or Alexa Fluor® 647 (sc-5286 AF647), 200 µg/ml, for IF, IHC(P) and FCM.
Answered by: Tech Service 5
Date published: 2016-12-15
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Rated 5 out of 5 by from Strong IF signals This antibody gave a clean and strong IF signal in human melanoma cell line MDA-MB-435S and accompanying clones.
Date published: 2021-06-07
Rated 5 out of 5 by from alpha Tubulin antibody Very useful antibody for loading control in Western blotting.
Date published: 2021-02-10
Rated 4 out of 5 by from Great features We used alpha tubulin antibody for western blot 1:1000, in synaptosomal hippocampal rat brain fraction, and we got clear bands.
Date published: 2020-12-29
Rated 4 out of 5 by from Buono Ho ricevuto il free sample di questo anticorpo alcuni mesi fa. E' stato testato per alcuni western blot e ha funzionato bene.
Date published: 2020-11-05
Rated 5 out of 5 by from Great antibody Good antibody for WB. You can applied this antibody to various cell lines.
Date published: 2020-08-05
Rated 5 out of 5 by from Detecting of tubulin and microtubules Antibody sc-5286 works very well for Western blots for the detection of alpha-tubulin on nitrocellulose membranes. Antibody sc-5286 is also excellent for immunocytochemistry performed on formaldehyde-fixed cells to demarcate microtubules. It can be used for multilabelling experiments together with fluorescently tagged phalloidin. It is suitable for experiments that require quantitative image analysis.
Date published: 2020-02-15
Rated 5 out of 5 by from Good for Western blot The tubulin antibody works well for whole cell lysates on T24, Human UC cells
Date published: 2019-08-05
Rated 5 out of 5 by from great antibody We have been using this antibody for many years. It gives consistent results even being in the fridge for more than 4 years.
Date published: 2019-07-30
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