Anti-alpha Tubulin Antibody (B-5-1-2) recommended for detection of α Tubulin of multiple origin by WB, IP, IF and IHC(P)

Anti-α Tubulin Antibody (B-5-1-2): sc-23948

Anti-α Tubulin Antibody (B-5-1-2) is rated 4.9 out of 5 by 22.
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Datasheets

    • α Tubulin Antibody (B-5-1-2) is a mouse monoclonal IgG1 κ, cited in 419 publications, provided at 200 µg/ml
    • raised against Sarkosyl-resistant ribbons from Strongylocentrotus purpuratus (Sea Urchin) sperm axonemes
    • alpha Tubulin Antibody (B-5-1-2) is recommended for detection of α Tubulin of multiple origin by WB, IP, IF and IHC(P)
    • Anti-alpha Tubulin Antibody (B-5-1-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of α Tubulin (B-5-1-2): sc-23948.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP) are the preferred secondary detection reagents for α Tubulin Antibody (B-5-1-2) for WB and IHC(P) applications. These reagents are now offered in bundles with α Tubulin Antibody (B-5-1-2) (see ordering information below).

Has α Tubulin Antibody (B-5-1-2): sc-23948 been used for brain sections?

Asked by: Alex3
Thank you for your question. This antibody is fine for mouse tissue sections, and should work great on your mouse brain samples. If you have any further questions, please reach out to Technical support at [email protected], or via phone at 800-457-3801, ext 2 or via chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-12-04

I am working with mouse tissue, but need to perform immunofluorescence. Is this possible with α Tubulin Antibody (B-5-1-2)?

Asked by: Trav11
If working with mouse tissue, you will need to use one of our mouse monoclonal primary antibodies that are directly conjugated. The α Tubulin Antibody (B-5-1-2): sc-23948 is not currently available with direct conjugation. But, we have an alternative, α Tubulin Antibody (TU-02): sc-8035, that is available directly conjugated to HRP (sc-8035 HRP), phycoerythrin (sc-8035 PE), fluorescein (sc-8035 FITC), Alexa Fluor® 488 (sc-8035 AF488) or Alexa Fluor® 647 (sc-8035 AF647). This product can be found at the following link: https://www.scbt.com/scbt/product/alpha-tubulin-antibody-tu-02
Answered by: Tech Service 8
Date published: 2016-12-22
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Rated 5 out of 5 by from A bit weaker than DM1A but equally good Excellent for WB and IF, using saponin for permeabilization in IF.
Date published: 2020-11-20
Rated 5 out of 5 by from Work well as internal control The band developed in WB was clear and consistent. We are satisfactory for its performance as an internal control.
Date published: 2020-09-01
Rated 5 out of 5 by from Wonderful housekeeping gene, loading control Consistently smooth, clean bands at 55kDa are observed, serving as a loading control and marker of cytoskeleton elements (microtubule) in various human cell lines.
Date published: 2018-07-12
Rated 5 out of 5 by from good antibody I tried it for IF. Good signals although they were slightly weaker than DM1A's.
Date published: 2018-06-18
Rated 5 out of 5 by from confirmed for ICC, IHC, WB This antibody works very well for immunocytochemistry, immunohistochemistry (frozen sections) and western blots.
Date published: 2017-11-12
Rated 5 out of 5 by from Used in WB strong signal for WB, we usually use the dilution 1:500
Date published: 2017-08-17
Rated 4 out of 5 by from Reliable loading control Good antibody for western blot in cell lysates. It has specific and clear bands that are easy to quantificate. It is also a reliable loading control.
Date published: 2017-05-30
Rated 5 out of 5 by from good quality antibody This antibody was used to detect alpha Tubulin antibody in human brain tissue , It worked very well in WB, very satisfied.
Date published: 2017-05-23
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