Anti-alpha Tubulin Antibody (AA12) recommended for detection of α Tubulin of mouse, rat and human origin by WB and IP

Anti-α Tubulin Antibody (AA12): sc-58667

Anti-α Tubulin Antibody (AA12) is rated 4.5 out of 5 by 2.
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Datasheets

    • α Tubulin Antibody (AA12) is a mouse monoclonal IgG2a κ, cited in 38 publications, provided at 200 µg/ml
    • raised against synthetic α Tubulin of human origin
    • recommended for detection of α Tubulin of mouse, rat and human origin by WB and IP
    • See α Tubulin (TU-02): sc-8035 for α Tubulin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred secondary detection reagent for α Tubulin Antibody (AA12) for WB applications. This reagent is now offered in a bundle with α Tubulin Antibody (AA12) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

Are there any issues with using sc-58667: α Tubulin (AA12) mouse monoclonal primary antibody on mouse samples?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-58667 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by from Published Clone The AA12 clone is well published for use in Western Blot for detecting alpha tubulin.
Date published: 2017-02-07
Rated 4 out of 5 by from Good WB data collected in rat brain Good WB data collected in rat brain. -SCBT QC
Date published: 2013-09-24
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