Anti-alpha Tubulin Antibody (A-6) recommended for detection of α Tubulin of mouse, rat, human and avian origin by WB, IP, IF and ELISA

Anti-α Tubulin Antibody (A-6): sc-398103

Anti-α Tubulin Antibody (A-6) is rated 5.0 out of 5 by 3.
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Datasheets

    • α Tubulin Antibody (A-6) is a mouse monoclonal IgG2b κ, cited in 43 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 22-49 N-terminus (h) of α Tubulin of human origin
    • alpha Tubulin Antibody (A-6) is recommended for detection of α Tubulin of mouse, rat, human and avian origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
    • Anti-alpha Tubulin Antibody (A-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of α Tubulin (A-6): sc-398103.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred secondary detection reagent for α Tubulin Antibody (A-6) for WB applications. This reagent is now offered in a bundle with α Tubulin Antibody (A-6) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

En qué se diluye la alfa tubulina en BSA o en aLECHE

Asked by: JoseB
Thank you for your question. We recommend dilution the antibody in 5% milk with TBS-T. You can find the recommended protocol here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Tech Service
Date published: 2019-05-03

I am using α Tubulin Antibody (A-6): sc-398103 for Western blot. How do I know if the band I am detecting is specific?

Asked by: jenni
We recommend using the blocking peptide sc-398103 P as a negative control. Antibody binding to antigen may be blocked/competed by pre-absorption with the blocking peptide. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support 8
Date published: 2017-01-06
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Rated 5 out of 5 by from An antibody you can count on! This product will produce the right results in your Western blot. If you are looking for the correct detection of alpha Tubulin, then there is no need to look any further. This antibody has been cited several times.
Date published: 2017-01-03
Rated 5 out of 5 by from Strong single band in A Strong single band in A-431 whole cell lysate. -SCBT QC
Date published: 2015-02-21
Rated 5 out of 5 by from Specific and sensitive for WB Specific and sensitive for WB, works in PC-12, K-562, MCF7, A549, BJAB and HeLa whole cell lysates. -SCBT QC
Date published: 2014-10-14
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