Anti-alpha Tubulin Antibody (3H3087) recommended for detection of α Tubulin of mouse, rat, human and yeast origin by WB, IP, IF and IHC(P)

Anti-α Tubulin Antibody (3H3087): sc-69971

Anti-α Tubulin Antibody (3H3087) is rated 4.5 out of 5 by 2.
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Datasheets

    • α Tubulin Antibody (3H3087) is a rat monoclonal IgG2a, cited in 9 publications, provided at 200 µg/ml
    • raised against full length purified α Tubulin of Saccharomyces cerevisiae origin
    • recommended for detection of α Tubulin of mouse, rat, human and yeast origin by WB, IP, IF and IHC(P); also recommended for detection of the tyrosinated form of α Tubulin
    • See α Tubulin (TU-02): sc-8035 for α Tubulin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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I am using α Tubulin (3H3087): sc-69971 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Excellent! This antibody works great in western blot analysis of α Tubulin expression in A549 and A-431 whole cell lysates.
Date published: 2017-02-01
Rated 4 out of 5 by from Good WB data gathered in NIH/3T3 Good WB data gathered in NIH/3T3, PC-12 and K-562 whole cell lysates. -SCBT QC
Date published: 2013-04-07
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