Anti-alpha Tubulin Antibody (10D8) recommended for detection of α Tubulin of broad species origin by WB, IP, IF and ELISA

Anti-α Tubulin Antibody (10D8): sc-53646

Anti-α Tubulin Antibody (10D8) is rated 5.0 out of 5 by 11.
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Datasheets

    • α Tubulin Antibody (10D8) is a mouse monoclonal IgM κ, cited in 145 publications, provided at 200 µg/ml
    • raised against full-length α Tubulin of human origin
    • recommended for detection of α Tubulin of broad species origin by WB, IP, IF and ELISA
    • See α Tubulin (TU-02): sc-8035 for α Tubulin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of α Tubulin (10D8): sc-53646.

What is the recommended fixation method for IF with this antibody?

Asked by: cjMara
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support 12
Date published: 2017-01-09
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Rated 5 out of 5 by from Good antibody! This antibody works very well with specific bands in WB, at 1:2000 dilution.
Date published: 2021-01-26
Rated 5 out of 5 by from works well works well, detects single band around 55kDa. Dilution was 1:2000.
Date published: 2019-10-22
Rated 5 out of 5 by from the band is beatiful the tubulin band is specific and beautiful for wbanalysis
Date published: 2017-11-23
Rated 5 out of 5 by from Excellent antibody for western blotting Antibody detects a strong band on western blot for SKOV3 cell extracts.
Date published: 2017-09-19
Rated 5 out of 5 by from Nice WB results Observed nice strong bands across multiple samples by WB
Date published: 2017-01-18
Rated 5 out of 5 by from Outstanding Outstanding western blot and if staining results !
Date published: 2017-01-02
Rated 5 out of 5 by from This antibody worked very well in WB at 1:200 This antibody worked very well in WB at 1:200 dilution.
Date published: 2015-09-09
Rated 5 out of 5 by from Worked well in WB at 1:200 dilution Worked well in WB at 1:200 dilution.
Date published: 2015-05-27
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