alpha-Adaptin 1/2 Antibody (100/2)recommended for detection of α-Adaptin 1 and α-Adaptin 2 of mouse, rat and human origin by WB and IP

α-Adaptin 1/2 Antibody (100/2): sc-58215

α-Adaptin 1/2 Antibody (100/2) is rated 4.0 out of 5 by 2.
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Datasheets

    • α-Adaptin 1/2 Antibody (100/2) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against AP2 polypeptides from bovine brain
    • recommended for detection of α-Adaptin 1 and α-Adaptin 2 of mouse, rat and human origin by WB and IP; also reactive with additional species, including bovine
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See α-Adaptin 1/2 (C-8): sc-17771 for α-Adaptin 1/2 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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How much sample do you recommend loading on the gel for Western blot detection using α-Adaptin 1/2 (100/2): sc-58215?

Asked by: Trav11
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Strong band in WB in HepG2 Strong band in WB in HepG2, NIH/3T3 and c4 whole cell lysates. -SCBT QC
Date published: 2015-02-10
Rated 4 out of 5 by from IP IP, WB publishable data with human Hela - SCBT Publication Review
Date published: 2014-02-16
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