ALK Antibody (F-12)recommended for detection of ALK of mouse, rat and human origin by WB, IP, IF and ELISA

ALK Antibody (F-12): sc-398791

ALK Antibody (F-12) is rated 4.9 out of 5 by 7.
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Datasheets

    • ALK Antibody (F-12) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 117-145 within an N-terminal extracellular domain of ALK of human origin
    • ALK Antibody (F-12) is recommended for detection of ALK of mouse, rat and human origin by WB, IP, IF and ELISA
    • ALK Antibody (F-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-398791 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ALK (F-12): sc-398791.
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I want to know whether the antibody (ALK Antibody (F-12): sc-398791) conjugated HRP can be used to examine the ALK rearrangement. Thank you!

Asked by: Leo1234
Thank you for your question. The HRP conjugated antibody is recommended for for WB, IHC(P) and ELISA. It is unclear what your intended research application is that you refer to as examining the ALK rearrangment. Please contact our Technical Service Department for further support.
Answered by: Technical Support
Date published: 2018-12-06

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with ALK Antibody (F-12): sc-398791?

Asked by: Trav11
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 15
Date published: 2017-01-25
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Rated 4 out of 5 by from Great features I use this antibody on pancreatic cancer cell line and have good bands.
Date published: 2018-03-14
Rated 5 out of 5 by from Good signal I used it for WB at 1:500 dilution, specific and clean signal
Date published: 2017-10-24
Rated 5 out of 5 by from Great feature! I used it in WB assay at 1:1000 and it works very well
Date published: 2017-07-12
Rated 5 out of 5 by from excellent antibody for westerns probed at 1/500X dilution for 90min and used in conjunction with the Kappa-binding-HRP as secondary and the blot turned out perfect. Excellent antibody for western blotting.
Date published: 2017-06-23
Rated 5 out of 5 by from Excellent for WB The (F-12) clone gives robust, clean and specific results in WB. An excellent choice!
Date published: 2017-01-12
Rated 5 out of 5 by from Produced positive Western Blot data of ALK Produced positive Western Blot data of ALK expression in HuT 78, U-937, Raji, K-562, MDA-MB-231 and MDA-MB-435S whole cell lysates. -SCBT QC
Date published: 2015-01-27
Rated 5 out of 5 by from Nice Western Blot data of ALK expression Nice Western Blot data of ALK expression in Jurkat whole cell lysate. -SCBT QC
Date published: 2013-08-03
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