Alix (h): 293 Lysate: sc-113330

Alix (h): 293 Lysate is derived from the 293 cell line, a human embryonic kidney cell line widely used in molecular and cellular biology research due to its reliable growth and transfection efficiency. This lysate is enriched with human ALG-2 interacting protein X (Alix), a multifunctional protein involved in various cellular processes, including endosomal sorting, membrane trafficking, and apoptosis. Alix is known for its role in the Endosomal Sorting Complex Required for Transport (ESCRT) pathway, where it facilitates the budding and scission of intraluminal vesicles within multivesicular bodies. In research, Alix (h): 293 Lysate is employed to study the molecular mechanisms underlying membrane dynamics and vesicular trafficking. Researchers use this lysate in Western blotting to detect and quantify Alix expression levels, providing insights into its regulation under different experimental conditions. The lysate is also utilized in co-immunoprecipitation assays to identify protein-protein interactions involving Alix, helping to explain its role in the ESCRT pathway and other cellular functions. Additionally, the lysate is valuable in experiments aimed at understanding Alix's involvement in apoptosis and its interaction with apoptosis-linked proteins, such as ALG-2 and TSG101. This research contributes to a deeper understanding of how Alix coordinates cellular processes related to membrane trafficking and programmed cell death. The origin and quality of the 293 cell line are rigorously controlled to ensure consistency and reproducibility in experimental outcomes, making Alix (h): 293 Lysate a reliable tool for studying fundamental biological mechanisms in cellular research.