ALDH1/2 Antibody (H-8)recommended for detection of ALDH1A1, ALDH1A2, ALDH1A3 and ALDH2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

ALDH1/2 Antibody (H-8): sc-166362

ALDH1/2 Antibody (H-8) is rated 4.7 out of 5 by 26.
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Datasheets

    • ALDH1/2 Antibody (H-8) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 186-270 mapping within an internal region of ALDH1A1 of human origin
    • ALDH1/2 Antibody (H-8) is recommended for detection of ALDH1A1, ALDH1A2, ALDH1A3 and ALDH2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • ALDH1/2 Antibody (H-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ALDH1/2 (H-8): sc-166362.
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What is the recommended fixation method for IF with ALDH1/2 (H-8): sc-166362 monoclonal antibody?

Asked by: jenni
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support 3
Date published: 2017-01-10
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Rated 5 out of 5 by from 该抗体效价比高,很好发 我两个月前申请该抗体,1:1000发光,Western 曝光时间短,条带清晰,效果很满意,值得推荐!
Date published: 2018-04-05
Rated 5 out of 5 by from Good reaction on paraffin mouse sections We bought this antibody in November 2017, but we managed to do the IHC only recently. We performed IHC on paraffin sections of mouse urinary bladder tissue. We used MW antigen retrieval with TRIS/EDTA buffer. The reaction was very good
Date published: 2018-01-09
Rated 5 out of 5 by from Good signal. Results are very clean. I bought this antibody a couple months ago. I tested it with several breast cancer lines. I am very happy with it.
Date published: 2017-12-14
Rated 4 out of 5 by from Works very well on mouse liver WB Works very well on mouse liver lysates western blotting, very clean and clear bands.
Date published: 2017-10-10
Rated 5 out of 5 by from ALDH1/2 Excelent antibody for western blot. Detected ALDH1/2 at expected size in 15 ug total proteine lysate, wet transfer one hour at 450 mAmps on PVDF membrane, overnight blocking with 5% nonfat dry milk in PBS, one hour incubation at RT with 1:500 anti ALDH1/2 in 5% PBS with 0,2%Tween followed by one hour incubation with 1:10.000 anti mouse (sc-358914) HRP conjugated antibody and detection with ECL prime substrate followed by short film exposure (under 1 minute) and automatic processing.
Date published: 2017-09-25
Rated 4 out of 5 by from Produced well The result was very clear,specific and strong by WB.
Date published: 2017-08-24
Rated 5 out of 5 by from perfect I applied this antibody sample several days ago.It was diluted to 1:500,primary antibody was incubated overnight at 4℃.I am that the band is clear
Date published: 2017-08-15
Rated 3 out of 5 by from Some Great Features! Blocked in 5% BSA-TBST 1:1000. Incubated in primary overnight in cold room. Tested on Murine Fallopian Tube Tissue and Human Ovarian Cell Lines. Worked in both but there are a lot of non-specific bands.
Date published: 2017-07-24
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