Anti-ALB/Albumin Antibody (F-8) recommended for detection of ALB of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA

Anti-ALB/Albumin Antibody (F-8): sc-374670

Anti-ALB/Albumin Antibody (F-8) is rated 4.7 out of 5 by 14.
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Datasheets

    • ALB/Albumin Antibody (F-8) is a mouse monoclonal IgG2a κ, cited in 7 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 559-591 near the C-terminus of serum albumin of mouse origin
    • Anti-ALB/Albumin Antibody (F-8) is recommended for detection of ALB of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-ALB/Albumin Antibody (F-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ALB/Albumin (F-8): sc-374670.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for ALB/Albumin Antibody (F-8) for WB and IHC(P) applications. These reagents are now offered in bundles with ALB/Albumin Antibody (F-8) (see ordering information below).
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If I block paraffin-embedded sections with 2% Bovine Serum Albumin (BSA), then stain with this antibody, will it recognize BSA? or it is very specific to mouse albumin? Thank you for your help, Patrick

Asked by: patrickaouad
Thank you for your question. We recommend using UltraCruz® Blocking Reagent: sc-516214 for blocking, especially when working with ALB Antibody (F-8): sc-374670. If you have further questions please contact our technical service.
Answered by: SCBT Heidelberg Support
Date published: 2019-12-20

What application is the blocking peptide sc-374670 P appropriate for?

Asked by: Dr Ninau Qelp
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2019-12-20
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Rated 5 out of 5 by from Great Antibody for Albumin detection I obtained a free aliquot to try this albumin antibody. It works great with IHC.
Date published: 2018-03-11
Rated 5 out of 5 by from Works great on WB Tried this antibody for WB on mouse tissue. It gave very strong signal at x1000 and worked great.
Date published: 2017-09-27
Rated 5 out of 5 by from good quality ALB antibody 1:800 detect rat liver tissue extracts (80ug) was analyzed by Western blot.Strong band at 67kda,very specific and no background.
Date published: 2017-09-16
Rated 5 out of 5 by from Nice ALB band I got this sample antibody to test the BBB integrity of mouse brain. dilute at (1:500), it works well, incubates at 4 degrees overnight. the result shows the strong and nice band at 67 kD.
Date published: 2017-05-10
Rated 5 out of 5 by from Working great Rat liver tissue is really positive, 1:200 for IHC, we use it often
Date published: 2017-05-07
Rated 5 out of 5 by from used in WB I have used this antibody for western blots at 1:1000 in 5% milk, the data is really amazing.
Date published: 2017-04-05
Rated 5 out of 5 by from Good antibody for WB! This antibody is great for WB, i got strong band and clean background!
Date published: 2017-03-08
Rated 5 out of 5 by from Excellent! This antibody works great in western blot analysis of ALB expression in mouse liver tissue extract.
Date published: 2017-01-17
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